Anti-LATS1 +LATS2 (phospho T1079 + T1041)抗体(ab111344)

概述

  • 产品名称
    Anti-LATS1 +LATS2 (phospho T1079 + T1041)抗体
    参阅全部 LATS1 +LATS2 一抗
  • 描述
    兔多克隆抗体to LATS1 +LATS2 (phospho T1079 + T1041)
  • 特异性
    ab111344 detects endogenous levels of LATS1 +LATS2 only when phosphorylated at threonine 1079/1041.
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from C-terminal region of Human LATS1 +LATS2 around the phosphorylation site of threonine 1079/1041 (E-F-TP-F-R).

  • 阳性对照
    • Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab111344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性
    Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.
  • 细胞定位
    Cytoplasm; cytoskeleton; centrosome.
  • 数据库链接
  • 别名
    • 4932411G09Rik antibody
    • AV277261 antibody
    • AW208599 antibody
    • AW228608 antibody
    • FLJ13161 antibody
    • KPM antibody
    • Large tumor supressor, homolog 1 antibody
    • LATS, large tumor suppressor, homolog 1 (Drosophila) antibody
    • LATS, large tumor suppressor, homolog 2 (Drosophila) antibody
    • RGD1564085 antibody
    • Serine/threonine protein kinase LATS1 antibody
    • Serine/threonine protein kinase LATS2 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all

图片

  • ab111344 at 1/50 dilution staining LATS1 +LATS2 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

文献

ab111344 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry
Sample
Human Cultured Cells (immortalised glioma cell lines U343 and U87)
Permeabilization
Yes - 0.1% Triton-X-100
Specification
immortalised glioma cell lines U343 and U87
Fixative
Paraformaldehyde
Username

Katherine Eales

Verified customer

提交于 Nov 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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