概述

  • 产品名称Anti-LATS1抗体
    参阅全部 LATS1 一抗
  • 描述
    兔多克隆抗体to LATS1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 971-1020 (IPPQAKLSPE ASDLIIKLCR GPEDRLGKNG ADEIKAHPFF KTIDFSSDLR) of Human LATS1 (NP_004681).

  • 阳性对照
    • THP1 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab99006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 127 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
  • 组织特异性Expressed in all adult tissues examined except for lung and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • 翻译后修饰Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
  • 细胞定位Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • h-warts antibody
    • Large tumor suppressor homolog 1 antibody
    • LATS large tumor suppressor homolog 1 antibody
    • LATS1 antibody
    • LATS1_HUMAN antibody
    • Serine threonine protein kinase LATS1 antibody
    • Serine/threonine-protein kinase LATS1 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all

Anti-LATS1 antibody 图像

  • Anti-LATS1 antibody (ab99006) at 1 µg/ml + THP1 cell lysate at 10 µg

    Predicted band size : 127 kDa

Anti-LATS1 antibody (ab99006)参考文献

ab99006 has not yet been referenced specifically in any publications.

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