Customer reviews and FAQs

概述

  • 产品名称
    Anti-LATS1抗体
    参阅全部 LATS1 一抗
  • 描述
    兔多克隆抗体to LATS1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1075 and the C terminus (residue 1130) of human LATS1 (SwissProt: O95835)

  • 阳性对照
    • HeLa whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
  • 组织特异性
    Expressed in all adult tissues examined except for lung and kidney.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • 翻译后修饰
    Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
  • 细胞定位
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • h-warts antibody
    • Large tumor suppressor homolog 1 antibody
    • LATS large tumor suppressor homolog 1 antibody
    • LATS1 antibody
    • LATS1_HUMAN antibody
    • Serine threonine protein kinase LATS1 antibody
    • Serine/threonine-protein kinase LATS1 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all

Anti-LATS1 antibody 图像

  • Western blot - LATS1 antibody (ab70562)
    Western blot - LATS1 antibody (ab70562)
    All lanes : Anti-LATS1 antibody (ab70562) at 0.4 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg

    Developed using the ECL technique

    Predicted band size : 127 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 350 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • Immunoprecipitation - LATS1 antibody (ab70562)
    Immunoprecipitation - LATS1 antibody (ab70562)
    Detection of human LATS1 by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded per lane), using ab70562 at 3 µg/mg of lysate (lane 3). LATS1 was also immunoprecipitated with ab70562 (lane 1) and with an other LATS1 antibody (lane 2) using 3 µg/mg lysate. Lane 4: Control IgG immunoprecipitate.
    Subsequent Western blot detection of LATS1 was performed using ab70562 at 0.4 µg/ml.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-LATS1 antibody (ab70562)参考文献

This product has been referenced in:
  • Liu S  et al. miR-21 modulates resistance of HR-HPV positive cervical cancer cells to radiation through targeting LATS1. Biochem Biophys Res Commun 459:679-85 (2015). WB ; Human . Read more (PubMed: 25769949) »

See 1 Publication for this product

发表研究结果有使用 ab70562？请让我们知道，以便我们可以引用本数据表中的参考文章。

Product Wall

提交 ab70562 的 abreview 提交 ab70562 的问题

1 Abreview or Q&A

10
Votes: Highest First

Western blot abreview for Anti-LATS1 antibody

 Average
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Dissociated hippocampal neurons)
Gel Running Conditions
Reduced Denaturing (8 % SDS-PAGE)
Loading amount
15 µg
Specification
Dissociated hippocampal neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
abreview image
Username

Mrs. Crystal Milligan

Verified customer

提交于 Aug 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册