概述

  • 产品名称Anti-LATS1抗体
    参阅全部 LATS1 一抗
  • 描述
    兔多克隆抗体to LATS1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Dog, Chimpanzee, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to a region between residues 50 and 100 of human LATS1 (SwissProt: O95835).

  • 阳性对照
    • Whole cell lysate from HeLa Cells.

性能

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应用

Our Abpromise guarantee covers the use of ab70561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/15000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
  • 组织特异性Expressed in all adult tissues examined except for lung and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • 翻译后修饰Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
  • 细胞定位Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • h-warts antibody
    • Large tumor suppressor homolog 1 antibody
    • LATS large tumor suppressor homolog 1 antibody
    • LATS1 antibody
    • LATS1_HUMAN antibody
    • Serine threonine protein kinase LATS1 antibody
    • Serine/threonine-protein kinase LATS1 antibody
    • WARTS antibody
    • WARTS protein kinase antibody
    • wts antibody
    see all

Anti-LATS1 antibody 图像

  • All lanes : Anti-LATS1 antibody (ab70561) at 0.02 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg


    Predicted band size : 127 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 117 kDa,200 kDa,268 kDa,71 kDa. We are unsure as to the identity of these extra bands.
  • Detection of human LATS1 by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70561 at 3 µg/mg of lysate (lane 1).
    LATS1 was also immunoprecipitated with an other LATS1 antibody (lanes 2) and with ab70562 (lane 3) using 3 µg/mg lysate.
    Lane 4: Control IgG immunoprecipitate.
    Subsequent Western blot detection of LATS1 was performed using ab70561 at 0.1 µg/ml.

Anti-LATS1 antibody (ab70561)参考文献

This product has been referenced in:
  • Goldie SJ  et al. FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis. Cancer Res 72:3424-36 (2012). Human . Read more (PubMed: 22564525) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (BEL-7402)
Gel Running Conditions Non-reduced Denaturing (10%)
Loading amount 40 µg
Treatment 40µM corosolic acid for 24hrs
Specification BEL-7402
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. guoqing zhu

Verified customer

提交于 Jan 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"