Anti-LAT2抗体(ab75610)

概述

  • 产品名称Anti-LAT2抗体
    参阅全部 LAT2 一抗
  • 描述
    兔多克隆抗体to LAT2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human LAT2.

    (Peptide available as ab90595.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates Human Liver Tissue; Mouse Kidney Tissue; Rat Kidney Tissue; Mouse Brain Tissue; HepG2 whole cell; HeLa whole cell.

性能

应用

Our Abpromise guarantee covers the use of ab75610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.
  • 组织特异性Strongest expression is observed in kidney and moderate expression in placenta and brain, followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell line Caco-2.
  • 序列相似性Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • 细胞定位Cytoplasm. Basolateral cell membrane. Localized to the cytoplasm when expressed alone but when coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hLAT2 antibody
    • integral membrane protein E16H antibody
    • L type amino acid transporter 2 antibody
    • L-type amino acid transporter 2 antibody
    • Large neutral amino acids transporter small subunit 2 antibody
    • LAT2_HUMAN antibody
    • LPI PC1 antibody
    • SLC7A8 antibody
    • solute carrier family 7 (amino acid transporter light chain, L system), member 8 antibody
    • solute carrier family 7 (amino acid transporter, L type), member 8 antibody
    • solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 antibody
    • Solute carrier family 7 member 8 antibody
    see all

Anti-LAT2 antibody 图像

  • All lanes : Anti-LAT2 antibody (ab75610) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 52 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • All lanes : Anti-LAT2 antibody (ab75610) at 1/1 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Kidney (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

Anti-LAT2 antibody (ab75610)参考文献

ab75610 has not yet been referenced specifically in any publications.

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