Anti-LAT2抗体(ab75610)
Key features and details
- Rabbit polyclonal to LAT2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-LAT2抗体
参阅全部 LAT2 一抗 -
描述
兔多克隆抗体to LAT2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Orangutan -
免疫原
Synthetic peptide corresponding to Human LAT2 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90595) -
阳性对照
- This antibody gave a positive signal in the following lysates Human Liver Tissue; Mouse Kidney Tissue; Rat Kidney Tissue; Mouse Brain Tissue; HepG2 whole cell; HeLa whole cell.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75610于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
靶标
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功能
Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. -
组织特异性
Strongest expression is observed in kidney and moderate expression in placenta and brain, followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell line Caco-2. -
序列相似性
Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. -
细胞定位
Cytoplasm. Basolateral cell membrane. Localized to the cytoplasm when expressed alone but when coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi. - Information by UniProt
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数据库链接
- Entrez Gene: 23428 Human
- Entrez Gene: 50934 Mouse
- Entrez Gene: 100009408 Rabbit
- Entrez Gene: 84551 Rat
- Omim: 604235 Human
- SwissProt: Q9UHI5 Human
- SwissProt: Q9QXW9 Mouse
- SwissProt: Q5RAE3 Orangutan
see all -
别名
- hLAT2 antibody
- integral membrane protein E16H antibody
- L type amino acid transporter 2 antibody
see all
图片
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All lanes : Anti-LAT2 antibody (ab75610) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 52 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
All lanes : Anti-LAT2 antibody (ab75610) at 1/1 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab75610 被引用在 7 文献中.
- Qi W & Zhang Q Identification and Validation of Immune Molecular Subtypes and Immune Landscape Based on Colon Cancer Cohort. Front Med (Lausanne) 9:827695 (2022). PubMed: 35602471
- Min Q et al. Genomic and epigenomic evolution of acquired resistance to combination therapy in esophageal squamous cell carcinoma. JCI Insight 6:N/A (2021). PubMed: 34494553
- McIntyre KR et al. Human placental uptake of glutamine and glutamate is reduced in fetal growth restriction. Sci Rep 10:16197 (2020). PubMed: 33004923
- Takakuwa A et al. Butyric Acid and Leucine Induce a-Defensin Secretion from Small Intestinal Paneth Cells. Nutrients 11:N/A (2019). PubMed: 31752111
- McIntyre KR et al. Evidence of adaptation of maternofetal transport of glutamine relative to placental size in normal mice, and in those with fetal growth restriction. J Physiol 597:4975-4990 (2019). PubMed: 31400764
- Dai W et al. Understanding the regulatory mechanisms of milk production using integrative transcriptomic and proteomic analyses: improving inefficient utilization of crop by-products as forage in dairy industry. BMC Genomics 19:403 (2018). PubMed: 29843597
- Gigena N et al. Dissecting thyroid hormone transport and metabolism in dendritic cells. J Endocrinol 232:337-350 (2017). PubMed: 28052998