概述

  • 产品名称Anti-LAT (phospho Y171)抗体
    参阅全部 LAT 一抗
  • 描述
    兔多克隆抗体to LAT (phospho Y171)
  • 特异性ab73205 detects endogenous levels of LAT only when phosphorylated at tyrosine 171.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human LAT around the phosphorylation site of tyrosine 171 (DDYPVN)

  • 阳性对照
    • Extracts from Jurkat cells treated with UV (5mins).

性能

应用

Our Abpromise guarantee covers the use of ab73205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 28 kDa).
ELISA 1/10000.

靶标

  • 功能Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • 组织特异性Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
  • 翻译后修饰Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
    Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
  • 细胞定位Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 36 kDa phospho tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adaptor protein antibody
    • LAT 1 antibody
    • lat antibody
    • LAT_HUMAN antibody
    • LAT1 antibody
    • Linker for activation of T cells antibody
    • Linker for activation of T cells family member 1 antibody
    • linker for activation of T cells, transmembrane adaptor antibody
    • Linker for activation of T-cells family member 1 antibody
    • p36 38 antibody
    • p36-38 antibody
    • pp36 antibody
    see all

Anti-LAT (phospho Y171) antibody 图像

  • All lanes : Anti-LAT (phospho Y171) antibody (ab73205) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with UV (5 min)
    Lane 2 : Extracts from Jurkat cells treated with UV (5 min) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

Anti-LAT (phospho Y171) antibody (ab73205)参考文献

This product has been referenced in:
  • Bronk CC  et al. NF-?B is crucial in proximal T-cell signaling for calcium influx and NFAT activation. Eur J Immunol N/A:N/A (2014). Read more (PubMed: 25251667) »
  • Driessens G  et al. Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-gamma1 phosphorylation. J Immunol 186:784-90 (2011). Read more (PubMed: 21149602) »

See all 2 Publications for this product

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