概述

  • 产品名称
  • 描述
    兔多克隆抗体to LAT
  • 特异性
    ab59229 detects endogenous levels of total LAT protein.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human LAT around the phosphorylation site of tyrosine 191 (R-E-YP-V-N).

  • 阳性对照
    • NIH 3T3 cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab59229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

靶标

  • 功能
    Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • 组织特异性
    Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
  • 翻译后修饰
    Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
    Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
  • 细胞定位
    Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 36 kDa phospho tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adapter protein antibody
    • 36 kDa phospho-tyrosine adaptor protein antibody
    • LAT 1 antibody
    • lat antibody
    • LAT_HUMAN antibody
    • LAT1 antibody
    • Linker for activation of T cells antibody
    • Linker for activation of T cells family member 1 antibody
    • linker for activation of T cells, transmembrane adaptor antibody
    • Linker for activation of T-cells family member 1 antibody
    • p36 38 antibody
    • p36-38 antibody
    • pp36 antibody
    see all

图片

  • All lanes : Anti-LAT antibody (ab59229) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extract
    Lane 2 : NIH 3T3 cell extract with immunising peptide


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

实验方案

文献

ab59229 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab59229.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册