概述

  • 产品名称Anti-LAT抗体
    参阅全部 LAT 一抗
  • 描述
    兔多克隆抗体to LAT
  • 特异性Specific for both forms of human LAT (36 and 38kDa).
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 31-233 of Human LAT.

  • 阳性对照
    • Jurkat lysate.

性能

应用

Our Abpromise guarantee covers the use of ab14518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: 4 µg immunoprecipitates LAT from 500 µg Jurkat RIPA lysate.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 36 kDa or 38 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
    • 组织特异性Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
    • 翻译后修饰Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
      Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
    • 细胞定位Cell membrane. Present in lipid rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 36 kDa phospho tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adaptor protein antibody
      • LAT 1 antibody
      • lat antibody
      • LAT_HUMAN antibody
      • LAT1 antibody
      • Linker for activation of T cells antibody
      • Linker for activation of T cells family member 1 antibody
      • linker for activation of T cells, transmembrane adaptor antibody
      • Linker for activation of T-cells family member 1 antibody
      • p36 38 antibody
      • p36-38 antibody
      • pp36 antibody
      see all

    Anti-LAT antibody (ab14518)参考文献

    ab14518 has not yet been referenced specifically in any publications.

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