The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: 4 µg immunoprecipitates LAT from 500 µg Jurkat RIPA lysate.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 36 kDa or 38 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.