- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-LASP1抗体
参阅全部 LASP1 一抗 -
描述山羊多克隆抗体to LASP1
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宿主Goat
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Chicken, Pig
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免疫原
Synthetic peptide:
NPNCARCGKIVYP
, corresponding to N terminal amino acids 2-14 of Human LASP1. -
阳性对照
- WB: Mouse brain extracts.
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常规说明Principal Names: LASP1 (LIM and SH3 protein 1), MLN50. GenBank Accession Number: NP006139 LocusLink ID: 3927 Please note that one of our customers has had problems getting this antibody to detect endogenous Lasp-1 in 2 different human cell lines. Furthermore, a LASP-1/GFP fusion protein overexpressed in a cell line could not be detected with this antibody and yet was detected with our Rabbit Polyclonal to GFP (ab290). For further information please see the enquiries section of the online datasheet. We are currently conducting more QC tests on this product and are interested to hear from other researchers using it. 24 March, 2004.
This protein functions as an actin-binding protein and possibly in cytoskeletal organization.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Primary antibody说明This protein functions as an actin-binding protein and possibly in cytoskeletal organization.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab1301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human LASP1 peptide (ab22798). |
靶标
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功能Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
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序列相似性Contains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain. -
细胞定位Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
- Information by UniProt
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数据库链接
- Entrez Gene: 420002 Chicken
- Entrez Gene: 3927 Human
- Entrez Gene: 16796 Mouse
- Entrez Gene: 29278 Rat
- Omim: 602920 Human
- SwissProt: Q14847 Human
- SwissProt: Q61792 Mouse
- SwissProt: P80171 Pig
see all -
别名
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
see all
图片
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Western blot - Anti-LASP1 antibody (ab1301)LASP1 staining (0.5µ g/ml) of Mouse Brain extracts (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence LASP1 staining (0.5µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence
文献
This product has been referenced in:
- Li K et al. Organelle proteomics of rat synaptic proteins: correlation-profiling by isotope-coded affinity tagging in conjunction with liquid chromatography-tandem mass spectrometry to reveal post-synaptic density specific proteins. J Proteome Res 4:725-33 (2005). Read more (PubMed: 15952719) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"