概述

  • 产品名称Anti-LASP1抗体
    参阅全部 LASP1 一抗
  • 描述
    山羊多克隆抗体to LASP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Pig
  • 免疫原

    Synthetic peptide:

    NPNCARCGKIVYP

    , corresponding to N terminal amino acids 2-14 of Human LASP1.

  • 阳性对照
    • WB: Mouse brain extracts.
  • 常规说明Principal Names: LASP1 (LIM and SH3 protein 1), MLN50. GenBank Accession Number: NP006139 LocusLink ID: 3927 Please note that one of our customers has had problems getting this antibody to detect endogenous Lasp-1 in 2 different human cell lines. Furthermore, a LASP-1/GFP fusion protein overexpressed in a cell line could not be detected with this antibody and yet was detected with our Rabbit Polyclonal to GFP (ab290). For further information please see the enquiries section of the online datasheet. We are currently conducting more QC tests on this product and are interested to hear from other researchers using it. 24 March, 2004.


    This protein functions as an actin-binding protein and possibly in cytoskeletal organization.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明This protein functions as an actin-binding protein and possibly in cytoskeletal organization.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human LASP1 peptide (ab22798).

靶标

Anti-LASP1 antibody 图像



  • Predicted band size : 30 kDa


    LASP1 staining (0.5µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

Anti-LASP1 antibody (ab1301)参考文献

This product has been referenced in:
  • Li K  et al. Organelle proteomics of rat synaptic proteins: correlation-profiling by isotope-coded affinity tagging in conjunction with liquid chromatography-tandem mass spectrometry to reveal post-synaptic density specific proteins. J Proteome Res 4:725-33 (2005). Read more (PubMed: 15952719) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (IMR-90 cells)
Loading amount 30 µg
Specification IMR-90 cells
Gel Running Conditions Non-reduced Denaturing
Username

Abcam user community

Verified customer

提交于 Apr 22 2009

Thank you for your email and I'm sorry to hear that you are experiencing some difficulty with these antibodies. All 3 of these antibodies have been successfully tested for application in Western blotting. However, these antibodies have not yet been...

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Thank you for your enquiry. I apologize for the confusion, the antibody is antigen affinity purified: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. If you have any ...

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I have asked our accounts department to refund you for the cost of this antibody. (Order reference 35289). Thank you for the extra information you have provided about the nature of your fusion constructs. We will follow your advice and have this a...

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Your feedback places doubt on the sensitivity/specificity of our antibody and we take it very seriously. As we discussed over the telephone, I will gladly refund you for the cost of this antibody as it clearly has not worked in your research. I'm afrai...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"