Anti-LAS1L抗体(ab49279)

概述

  • 产品名称Anti-LAS1L抗体
    参阅全部 LAS1L 一抗
  • 描述
    兔多克隆抗体to LAS1L
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse
  • 免疫原

    A region within synthetic peptide: PASSSFGSEA KAQQQEEQGS VNDVKEEEKE EKEVLPDQVE EEEENDDQEE, corresponding to C terminal amino acids 541-590 of Human LAS1L

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab49279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 83 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
  • 序列相似性Belongs to the LAS1 family.
  • 细胞定位Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Localizes mainly to the granular component, the region implicated in the later steps of rRNA processing and subunit assembly and export.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ475B7.2 antibody
    • FLJ12525 antibody
    • FLJ30133 antibody
    • FLJ45274 antibody
    • LAS1 like (S. cerevisiae) antibody
    • Las1 like antibody
    • LAS1L antibody
    • LAS1L_HUMAN antibody
    • Protein LAS1 homolog antibody
    • Ribosomal biogenesis protein LAS1L antibody
    • RP3 475B7.2 antibody
    see all

Anti-LAS1L antibody 图像

  • Anti-LAS1L antibody (ab49279) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit Ig at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 83 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

Anti-LAS1L antibody (ab49279)参考文献

ab49279 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (LUNG CANCER)
Loading amount 200000 cells
Specification LUNG CANCER
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 29 2009

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