概述

  • 产品名称Anti-LARP7抗体
    参阅全部 LARP7 一抗
  • 描述
    兔多克隆抗体to LARP7
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 533-582 (WQKILVDRQA KLNQPREKKR GTEKLITKAE KIRLAKTQQA SKHIRFSEYD) of Human LARP7 (NP_056269).

  • 阳性对照
    • This antibody gave a positive signal in HeLa whole cell lysate within WB and MCF7 cell line within IF/ICC. IHC-P (FFPE): Human Lung (Normal)

性能

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应用

Our Abpromise guarantee covers the use of ab105682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
  • 序列相似性Contains 1 HTH La-type RNA-binding domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp564K112 antibody
    • HDCMA18P antibody
    • La related protein 7 antibody
    • La ribonucleoprotein domain family member 7 antibody
    • La ribonucleoprotein domain family member 7 antibody
    • La-related protein 7 antibody
    • LARP 7 antibody
    • larp7 antibody
    • LARP7_HUMAN antibody
    • MGC104360 antibody
    • P TEFb interaction protein for 7SK stability antibody
    • P-TEFb-interaction protein for 7SK stability antibody
    • PIP7S antibody
    see all

Anti-LARP7 antibody 图像

  • ICC/IF image of ab105682 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105682, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-LARP7 antibody (ab105682) at 0.5 µg/ml + Hela cell lysate at 10 µg/ml

    Predicted band size : 67 kDa
    Gel concentration: 12%
  • IHC image of LARP7 staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105682, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-LARP7 antibody (ab105682)参考文献

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