This antibody does not cross-react with PHAPI or PHAP12a.
Mouse, Rat, Human
Synthetic peptide based on sequence near C-terminus (Human).
A549 or HepG2 whole cell lysate.
Stable for one year.
Shipped at 4°C. Store at +4°C.
Preservative: 0.02% Sodium Azide
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa.Can be blocked with
LANPL peptide (ab6242).
Use a concentration of 2 µg/ml.
Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity.
Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney.
Belongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats. Contains 1 LRRCT domain.
Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent.
Information by UniProt
Acidic (leucine rich) nuclear phosphoprotein 32 family member E antibody
Acidic leucine-rich nuclear phosphoprotein 32 family member E antibody
Western blot - LANPL antibody (ab5993)
Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with anti-PHAP III at 1
µg/ml. Western blot analysis of LANPL expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with ab5993 at 1 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LANPL antibody (ab5993)
ab5993 at 2µg/ml staining LANPL in human small intestine tissue by IHC
Immunocytochemistry/ Immunofluorescence-LANPL antibody(ab5993)
ICC/IF image of ab5993 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5993, 1µg/ml) overnight at +4°C. The secondary antibody (green) was
, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence-Anti-LANPL antibody(ab5993)
Immunofluorescence of PHAP III in Human Small Intestine cells using ab5993 at 20 ug/ml.
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