Anti-LAMP3抗体(ab83659)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to LAMP3
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT) of Human LAMP3 (NP_055213).

  • 阳性对照
    • MCF7 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 44, 64 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.
  • 组织特异性
    Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.
  • 序列相似性
    Belongs to the LAMP family.
  • 细胞定位
    Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD208 antibody
    • CD208 antigen antibody
    • DC LAMP antibody
    • DC lysosome associated membrane glycoprotein antibody
    • DC-lysosome-associated membrane glycoprotein antibody
    • DCLAMP antibody
    • LAMP antibody
    • LAMP 3 antibody
    • LAMP-3 antibody
    • Lamp3 antibody
    • LAMP3_HUMAN antibody
    • Lysosomal associated membrane protein 3 antibody
    • Lysosomal-associated membrane protein 3 antibody
    • Lysosome-associated membrane glycoprotein 3 antibody
    • Protein TSC403 antibody
    • TSC403 antibody
    see all

图片

  • Anti-LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44,64 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab83659 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us. LAMP3 is glycosylated and the higher MW band is likely a glycosylated form of the protein. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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