Anti-LAMP1抗体- Lysosome Marker (ab79821)

概述

  • 产品名称Anti-LAMP1抗体- Lysosome Marker
    参阅全部 LAMP1 一抗
  • 描述
    兔多克隆抗体to LAMP1 - Lysosome Marker
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Rabbit LAMP1.

    (Peptide available as ab25744.)

  • 阳性对照
    • This antibody gave a positive signal in the following formaldehyde fixed cell lines: HeLa; MCF-7.
  • 常规说明

    ab79821 is raised using the same immunogen as ab24170. Batches sold under ab79821 have passed QC testing in ICC/IF only. In our hands, this product did not work in WB. For further information please contact our Customer Services team.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab79821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Can be blocked with Human LAMP1 peptide (ab25744).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 序列相似性Belongs to the LAMP family.
  • 翻译后修饰O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 细胞定位Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Anti-LAMP1 antibody - Lysosome Marker 图像

  • All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab79821) at 1/2000 dilution

    Lane 1 : Normal Rat Cortical Lysate
    Lane 2 : Normal Human Cortical Lysate
    Lane 3 : Parkinson's disease dementia Cortical Lysate
    Lane 4 : Dementia with Lewy Bodies Cortical Lysate
    Lane 5 : Alzheimer's Disease Cortical Lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti Rabbit IgG conjugated to Alexa Fluor at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 120,44 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an abreview submitted by Dr. Martin Broadstock (King's College London, United Kingdom)

    Detection System: Odyssey System

    Blocking: Milk 5%

     

    See Abreview

  • ICC/IF image of ab79821 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79821, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml.

Anti-LAMP1 antibody - Lysosome Marker (ab79821)参考文献

ab79821 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (BA9 cortical sections)
Specification BA9 cortical sections
Permeabilization Yes - 0.05% Tween-20
Fixative Paraformaldehyde
Username

Dr. Martin Broadstock

Verified customer

提交于 Jun 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (BA9 cortical sections)
Specification BA9 cortical sections
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Martin Broadstock

Verified customer

提交于 Jun 19 2013

Thank you for your message. I apologize for the delay of mine! 1) There are no particular requirements for antibodies to work in flow cytometry, so the mentioned antibodies should give positive results using this technique. It is more important to o...

Read More

Thank you very much for your message. As I myself have been in the very same position, I very well understand that you and your supervisor hesitate to test our different CD107a antibodies. At that time I had the permission by my supervisor to test v...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Username

Abcam user community

Verified customer

提交于 Jul 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 May 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"