Anti-LAMP1抗体[LY1C6] (ab13523)


  • 产品名称Anti-LAMP1抗体[LY1C6]
    参阅全部 LAMP1 一抗
  • 描述
    小鼠单克隆抗体[LY1C6] to LAMP1
  • 经测试应用适用于: WB, IP, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster
  • 免疫原

    Rat liver lysosomal membrane preparations

  • 阳性对照
    • Highly purified rat lysosomal membrane preparations.
  • 常规说明For maximum product recovery centrifuge the product vial before removing cap.


Our Abpromise guarantee covers the use of ab13523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa.
IP Use at an assay dependent dilution.
ICC 1/100 - 1/750.
ICC/IF Use at an assay dependent concentration. PubMed: 21536677


  • 功能Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 序列相似性Belongs to the LAMP family.
  • 翻译后修饰O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 细胞定位Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Anti-LAMP1 antibody [LY1C6] 图像

  • ab13523 at 1/100 dilution staining LAMP1 in LAMP1 transfected HeLa cells by Immunocytochemistry/ immunofluorescence. A Alexa Fluor® 568 conjugated goat anti mouse was used at 1/50 dilution as secondary.

  • Image from Banno A et al, J Biol Chem. 2012 Apr 20;287(17):13799-812. Epub 2012 Feb 18, Fig 4. DOI 10.1074/jbc.M112.341214 April 20, 2012 The Journal of Biological Chemistry, 287, 13799-13812.

    ab13523 used in Western Blot. Lysates prepared from CHO cells stably expressing aIIbß3 integrins (A5 cells) were used.
    WCL, N/IC, C, P, and PM indicate whole cell lysates, nuclear/intact cell, cytosolic, high-speed pellet, and plasma membrane fractions, respectively.

Anti-LAMP1 antibody [LY1C6] (ab13523)参考文献

This product has been referenced in:
  • Wang B  et al. Essential control of mitochondrial morphology and function by chaperone-mediated autophagy through degradation of PARK7. Autophagy 12:1215-28 (2016). Read more (PubMed: 27171370) »
  • Sun H  et al. Resistance of glioma cells to nutrient-deprived microenvironment can be enhanced by CD133-mediated autophagy. Oncotarget 7:76238-76249 (2016). WB ; Rat . Read more (PubMed: 27780926) »

See all 13 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Sample Monkey Cell (cos7)
Specification cos7
Permeabilization Yes - 2% TritonX100
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Aug 13 2014

Thank you for your enquiry and your interest in our products. I believe my colleague has already provided a quote for you. Regarding the immunostaining - ab13523 is an unconjugated primary antibody so you would need a compatible seconda...

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Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (BHK 21)
Specification BHK 21
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.2%

Dr. Giuseppe Balistreri

Verified customer

提交于 Apr 05 2007

I think your protocol is very good and very similar to the one I have used for ICC, and have been investigating what successful methods were used with ab13523 in ICC. I conclude that I think the problem lies in the fixation step and would like to sugge...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Dog Cell lysate - whole cell (MDCK cell line)
Specification MDCK cell line
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%

Mrs. Kun Liu

Verified customer

提交于 Sep 02 2005

Thank you for your enquiry and for providing more information about your protocol. This antibody cross-reacts with rat and hamster LAMP1 but it has not been tested in other species. Unfortunately, we do not have any data indicating its suitability ...

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