Anti-LAMP1抗体- Lysosome Marker (ab30687)
Key features and details
- Rabbit polyclonal to LAMP1 - Lysosome Marker
- Suitable for: ICC
- Reacts with: Drosophila melanogaster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-LAMP1抗体- Lysosome Marker
参阅全部 LAMP1 一抗 -
描述
兔多克隆抗体to LAMP1 - Lysosome Marker -
宿主
Rabbit -
经测试应用
适用于: ICCmore details
不适用于: WB -
种属反应性
与反应: Drosophila melanogaster -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) LAMP1.
-
阳性对照
- ICC: Drosophila S2 Schneider cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab30687于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use a concentration of 0.2 µg/ml.
|
说明 |
---|
ICC
Use a concentration of 0.2 µg/ml. |
靶标
-
功能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
细胞定位
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
-
别名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
图片
-
ab30687 staining LAMP1 in Drosophila S2 Schneider cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab30687 at 0.2μg/ml concentration and ab44928 (Mouse monoclonal to Tubulin) at 0.2μg/ml concentration overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (23)
ab30687 被引用在 23 文献中.
- Tao X et al. Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells. JCI Insight 7:N/A (2022). PubMed: 35801587
- Linnemannstöns K et al. Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model. J Extracell Vesicles 11:e12263 (2022). PubMed: 36103151
- Sidisky JM et al. Mayday sustains trans-synaptic BMP signaling required for synaptic maintenance with age. Elife 10:N/A (2021). PubMed: 33667157
- Witte L et al. The kinesin motor Klp98A mediates apical to basal Wg transport. Development 147:N/A (2020). PubMed: 32665246
- Chittoor-Vinod VG et al. Dietary Amino Acids Impact LRRK2-Induced Neurodegeneration in Parkinson's Disease Models. J Neurosci 40:6234-6249 (2020). PubMed: 32605938