Anti-LAMP1抗体(ab62562)
Key features and details
- Rabbit polyclonal to LAMP1
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LAMP1抗体
参阅全部 LAMP1 一抗 -
描述
兔多克隆抗体to LAMP1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
A 15 amino acid peptide from near the centre of human LAMP1.
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阳性对照
- ICC: Human colon cells. WB: Mouse EL4 cell lysate. IHC-P: Rat colon tissue and human placenta tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62562于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 2 µg/ml.
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ICC |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa).
Western blot for mouse only |
说明 |
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IHC-P
Use a concentration of 2 µg/ml. |
ICC
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa). Western blot for mouse only |
靶标
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功能
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
细胞定位
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3916 Human
- Entrez Gene: 16783 Mouse
- Entrez Gene: 25328 Rat
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P11438 Mouse
- SwissProt: P14562 Rat
- Unigene: 494419 Human
see all -
别名
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
图片
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Immunocytochemical analysis of LAMP-1 in Human Colon cells using ab62562 at 20 ug/mL.
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Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml
All lanes : EL4 cell lysate
Predicted band size: 45 kDa -
Immunohistochemical analysis of LAMP-1 in rat colon tissue using ab62562 at 20µg/ml.
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Immunohistochemistry of LAMP-1 in rat colon tissue with ab62562 at 2.5 µg/ml.
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Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requi
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab62562 被引用在 20 文献中.
- Zhu Y et al. Targeting Aβ and p-Tau Clearance in Methamphetamine-Induced Alzheimer's Disease-Like Pathology: Roles of Syntaxin 17 in Autophagic Degradation in Primary Hippocampal Neurons. Oxid Med Cell Longev 2022:3344569 (2022). PubMed: 35633882
- Hyroššová P et al. Glycosylation defects, offset by PEPCK-M, drive entosis in breast carcinoma cells. Cell Death Dis 13:730 (2022). PubMed: 36002449
- Meecham A et al. Ligand-bound integrin αvβ6 internalisation and trafficking. Front Cell Dev Biol 10:920303 (2022). PubMed: 36092709
- Zhou L et al. Transcription factor EB-mediated autophagy promotes dermal fibroblast differentiation and collagen production by regulating endoplasmic reticulum stress and autophagy-dependent secretion. Int J Mol Med 47:547-560 (2021). PubMed: 33416091
- Hung C et al. SORL1 deficiency in human excitatory neurons causes APP-dependent defects in the endolysosome-autophagy network. Cell Rep 35:109259 (2021). PubMed: 34133918