概述

  • 产品名称Anti-LAMP1抗体
    参阅全部 LAMP1 一抗
  • 描述
    兔多克隆抗体to LAMP1
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 15 amino acid peptide from near the centre of human LAMP1.

  • 阳性对照
    • EL4 cell lysate. Human colon tissue.

应用

Our Abpromise guarantee covers the use of ab62562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 20 µg/ml.

靶标

  • 功能Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 序列相似性Belongs to the LAMP family.
  • 翻译后修饰O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 细胞定位Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Anti-LAMP1 antibody 图像

  • Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
    Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Despite its predicted size, LAMP1 migrates at 110kDa in SDS-PAGE.

    There are multiple additional bands. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry of LAMP-1 in rat colon tissue with ab62562 at 2.5 µg/ml.

  • Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requi
  • Immunofluorescence of LAMP-1 in Human Colon cells using ab62562 at 20 ug/mL.

Anti-LAMP1 antibody (ab62562)参考文献

This product has been referenced in:
  • Abramowski D  et al. Transgenic expression of intraneuronal Aß42 but not Aß40 leads to cellular Aß lesions, degeneration, and functional impairment without typical Alzheimer's disease pathology. J Neurosci 32:1273-83 (2012). ICC/IF ; Mouse . Read more (PubMed: 22279212) »
  • Jabbour M  et al. Discrete domains of MARCH1 mediate its localization, functional interactions, and posttranscriptional control of expression. J Immunol 183:6500-12 (2009). ICC/IF ; Mouse . Read more (PubMed: 19880452) »

See all 2 Publications for this product

Product Wall

The immunogen sequence is unfortunately proprietary information. The laboratory however made an alignement to predict crossreactivity. There is 66.7% homology with bovine [* ** * ** **** ]. The accession number used for the alignment was NP_001068592.1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"