概述

  • 产品名称
    Anti-LAMP1抗体[1D4B]
    参阅全部 LAMP1 一抗
  • 描述
    大鼠单克隆抗体[1D4B] to LAMP1
  • 特异性
    Murine CD107a/LAMP1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa)
  • 经测试应用
    适用于: IHC-P, ICC/IF, Other, Flow Cyt, IP, IHC-Fr, WB, Immunomicroscopymore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Plasma membrane fraction of mouse embryo NIH3T3 cell line.

  • 阳性对照
    • IHC-P: human lung FFPE tissue sections

应用

Our Abpromise guarantee covers the use of ab25245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 17215451
Other Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

AP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).
Immunomicroscopy Use at an assay dependent concentration.

靶标

  • 功能
    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • 序列相似性
    Belongs to the LAMP family.
  • 翻译后修饰
    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • 细胞定位
    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

图片

  • IHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25245, 10µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab25245 staining LAMP1 in Mouse epithelial cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with methanol/acetone 1:1 and blocked with 1% milk in PNB for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) in PNB for 16 hours at 4°C. An Alexa Fluor® 568-conjugated Donkey polyclonal to rat IgG, dilution 1/500, was used as secondary antibody. Nuclei were stained blue.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab25245 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25245, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab25245 staining mouse macrophage, hepatoma cell by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C.  An Alexa Fluor® 594 conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

文献

This product has been referenced in:
  • Sahu SK  et al. MicroRNA 26a (miR-26a)/KLF4 and CREB-C/EBPß regulate innate immune signaling, the polarization of macrophages and the trafficking of Mycobacterium tuberculosis to lysosomes during infection. PLoS Pathog 13:e1006410 (2017). Read more (PubMed: 28558034) »
  • Uchiyama K  et al. Prions amplify through degradation of the VPS10P sorting receptor sortilin. PLoS Pathog 13:e1006470 (2017). Read more (PubMed: 28665987) »

See all 34 Publications for this product

客户评价及客户问答

Thank you for contacting Abcam regarding ab25245.

I can confirm that according to SwissProt, the predicted molecular weight of LAMP1 is indeed ˜40kDa. However, this protein is subject to glycosylation at numerous site which accounts for the ...

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Thank you for contacting Abcam.

I have heard back from the about whether some of our LAMP1 antibodies recognize an extracellular epitope or not.

Although for ab25630 & ab25245, we have not performed epitope mapping; we have dete...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Apr 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

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提交于 Apr 05 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 05 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Apr 05 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 24 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain (microgliosis neocortex))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization
Yes - Tween20
Specification
brain (microgliosis neocortex)
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
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提交于 Feb 10 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse macrophage)
Permeabilization
No
Specification
mouse macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

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Verified customer

提交于 Oct 30 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Sample
Mouse Cell (Neuronal progenitor cell line)
Specification
Neuronal progenitor cell line
Permeabilization
Yes - PBS 1% BSA 0,1% Saponine
Fixative
Paraformaldehyde
Username

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Verified customer

提交于 Apr 08 2014

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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