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Plasma membrane fraction of mouse embryo NIH3T3 cell line.
Our Abpromise guarantee covers the use of ab25245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17215451|
|Other||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|AP||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).|
|Immunomicroscopy||Use at an assay dependent concentration.|
ab25245 staining mouse macrophage, hepatoma cell by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 594 conjugated goat anti-mouse antibody was used as the secondary.