Anti-Laminin gamma 1抗体[SPM277] (ab17792)

概述

  • 产品名称Anti-Laminin gamma 1抗体[SPM277]
    参阅全部 Laminin gamma 1 一抗
  • 描述
    大鼠单克隆抗体[SPM277] to Laminin gamma 1
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Tissue/ cell preparation. (Murine EHS laminin preparation)

  • 阳性对照
    • LS174T cells. Normal colon or colon carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab17792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 210 kDa. The best results were obtained by incubating the primary antibody overnight at 4C.
IP Use at 2 µg/mg of lysate.
IHC-P 1/50. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000 U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37C.

靶标

  • 功能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 组织特异性Found in the basement membranes (major component).
  • 序列相似性Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • 结构域The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LAMB2 antibody
    • lamc1 antibody
    • LAMC1_HUMAN antibody
    • Laminin B2 chain antibody
    • Laminin subunit gamma-1 antibody
    • Laminin, gamma 1 (formerly LAMB2) antibody
    • Laminin-1 subunit gamma antibody
    • Laminin-10 subunit gamma antibody
    • Laminin-11 subunit gamma antibody
    • Laminin-2 subunit gamma antibody
    • Laminin-3 subunit gamma antibody
    • Laminin-4 subunit gamma antibody
    • Laminin-6 subunit gamma antibody
    • Laminin-7 subunit gamma antibody
    • Laminin-8 subunit gamma antibody
    • Laminin-9 subunit gamma antibody
    • MGC87297 antibody
    • S-LAM gamma antibody
    • S-laminin subunit gamma antibody
    see all

Anti-Laminin gamma 1 antibody [SPM277] 图像

  • ab17792 at 1/50 staining Laminin gamma 1 from human tonsil by immunohistochemistry
  • ab17792 staining Laminin gamma 1 in dog skeletal muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone. Samples were incubated with the primary antibody at a 1/100 dilution for 8 hours at 4°C. An FITC-conjugated goat polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

Anti-Laminin gamma 1 antibody [SPM277] (ab17792)参考文献

This product has been referenced in:
  • Fournier B  et al. Blockade of the activin receptor IIb activates functional brown adipogenesis and thermogenesis by inducing mitochondrial oxidative metabolism. Mol Cell Biol 32:2871-9 (2012). Read more (PubMed: 22586266) »
  • Steenhard BM  et al. Transgenic Expression of Human LAMA5 Suppresses Murine Lama5 mRNA and Laminin a5 Protein Deposition. PLoS One 6:e23926 (2011). WB ; Mouse . Read more (PubMed: 21915268) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Kidney)
Permeabilization Yes - 15 min with 0.1% Triton
Specification Kidney
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 24 2016

Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative Acetone
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Oct 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"