The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 210 kDa. The best results were obtained by incubating the primary antibody overnight at 4C.
Use at 2 µg/mg of lysate.
1/50. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000 U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37C.
功能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
组织特异性Found in the basement membranes (major component).
序列相似性Contains 11 laminin EGF-like domains. Contains 1 laminin IV type A domain. Contains 1 laminin N-terminal domain.
结构域The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. Domains VI and IV are globular.
细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
ab17792 at 1/50 staining Laminin gamma 1 from human tonsil by immunohistochemistry
Immunohistochemistry (Frozen sections) - Laminin gamma 1 antibody [SPM277] (ab17792)Image courtesy of an anonymous Abreview.
ab17792 staining Laminin gamma 1 in dog skeletal muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone. Samples were incubated with the primary antibody at a 1/100 dilution for 8 hours at 4°C. An FITC-conjugated goat polyclonal was used as secondary antibody at a 1/200 dilution.
Fournier B et al. Blockade of the activin receptor IIb activates functional brown adipogenesis and thermogenesis by inducing mitochondrial oxidative metabolism. Mol Cell Biol32:2871-9 (2012).
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Steenhard BM et al. Transgenic Expression of Human LAMA5 Suppresses Murine Lama5 mRNA and Laminin a5 Protein Deposition. PLoS One6:e23926 (2011).
Read more (PubMed: 21915268) »