Anti-Laminin gamma 1抗体[BC17] (ab41823)
Key features and details
- Mouse monoclonal [BC17] to Laminin gamma 1
- Suitable for: ELISA, IHC-Fr, IP
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-Laminin gamma 1抗体[BC17]
参阅全部 Laminin gamma 1 一抗 -
描述
小鼠单克隆抗体[BC17] to Laminin gamma 1 -
宿主
Mouse -
特异性
The antibody recognizes the gamma 1 chain of human reduced and alkylated laminin produced by various cultured cells, which produce laminins complexed with it. The specificity of clone BC17 has been confirmed by immunoprecipitation and ELISA by using recombinant Laminin gamma 1. -
经测试应用
适用于: ELISA, IHC-Fr, IPmore details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
Pepsin-digested laminin from human placenta.
-
常规说明
Balb/c spleen cells used to produce hybridoma.The monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
纯度
Protein G purified -
Primary antibody说明
The monoclonal antibody BC17 against Laminin gamma 1 can be widely applied in immunohistochemical studies as all basement membranes contain laminin trimers containing this chain. Antibodies to laminin chains are useful in basic biology studies as well as in pathological studies concerning e.g. the integrity of the basement membranes in carcinomas. -
克隆
单克隆 -
克隆编号
BC17 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41823于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
说明 |
---|
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
靶标
-
功能
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
组织特异性
Found in the basement membranes (major component). -
序列相似性
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
结构域
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3915 Human
- Omim: 150290 Human
- SwissProt: P11047 Human
- SwissProt: Q5VYE7 Human
- SwissProt: Q6NVY8 Human
- Unigene: 609663 Human
-
别名
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
see all
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab41823 被引用在 1 文献中.
- Geberhiwot T et al. Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains. Matrix Biol 19:163-7 (2000). PubMed: 10842099