Anti-Laminin gamma 1抗体[A5] - BSA and Azide free (ab80580)

概述

  • 产品名称Anti-Laminin gamma 1抗体[A5] - BSA and Azide free
    参阅全部 Laminin gamma 1 一抗
  • 描述
    大鼠单克隆抗体[A5] to Laminin gamma 1 - BSA and Azide free
  • 特异性This antibody uniformly stains all human and murine basement membranes.
  • 经测试应用适用于: IP, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Murine EHS laminin preparation.

  • 阳性对照
    • LS174T cells. Normal colon or colon carcinoma.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab80580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
IHC-P
IHC-Fr
ICC/IF
  • 应用说明ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 µg/ml.
    IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
    IP: Use at 2µg/mg of lysate. (Native and denatured)

    Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • 组织特异性Found in the basement membranes (major component).
    • 序列相似性Contains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • 结构域The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free 图像

    • Frozen human tonsil stained with ab80580 at 2µg/ml using peroxidase-conjugate and DAB chromogen. Note basement membrane and cell surface staining of blood vessels.
    • Native IP using LS174T cells:
      IP 2µg/mg lysate.
      WB at 2µg/ml.

    Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free (ab80580)参考文献

    This product has been referenced in:
    • Ljubimov AV  et al. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest 72:461-73 (1995). ICC/IF, IHC-P, IHC-Fr, IP ; Human, Mouse . Read more (PubMed: 7723285) »
    • Ljubimov AV  et al. Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50:562-6 (1992). ICC/IF, IHC-P, IHC-Fr, IP ; Human, Mouse . Read more (PubMed: 1371500) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us. We can recommend using antibody ab106783, ab99667, ab99666 etc. Rabbit or goat anti rat IgG also cross reacts with IgG2a so anti rat IgGs can also be used. I hope this information is helpful to you. Please do not hesitate...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"