Anti-Laminin gamma 1抗体[A5] (Biotin) (ab79670)

概述

  • 产品名称Anti-Laminin gamma 1抗体[A5] (Biotin)
    参阅全部 Laminin gamma 1 一抗
  • 描述
    大鼠单克隆抗体[A5] to Laminin gamma 1 (Biotin)
  • 偶联物Biotin
  • 特异性ab79670 stains uniformly all human and murine basement membranes.
  • 经测试应用适用于: IP, IHC-Fr, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Murine EHS laminin preparation.

  • 阳性对照
    • LS174T cells; Normal colon, colon carcinoma and tonsil tissues.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab79670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
IHC-Fr
ICC/IF
IHC-P
  • 应用说明ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 µg/ml for 30 min at RT. Use Acid-alcohol-fixed tissue.
    Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37oC.
    IHC-Fr: Use at a concentration of 2 µg/ml for 30 min at RT. Use Acetone-fixed tissue.
    IP: Use at 2µg/mg of lysate (Native and denatured).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • 组织特异性Found in the basement membranes (major component).
    • 序列相似性Contains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • 结构域The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    Anti-Laminin gamma 1 antibody [A5] (Biotin) (ab79670)参考文献

    ab79670 has not yet been referenced specifically in any publications.

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