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Our Abpromise guarantee covers the use of ab44941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/200. Detects a band of approximately 210 kDa.|
|IHC-Fr||1/400. Use with acetone fixed tissues.|
|IHC (Methanol fixed)||1/400.|
|IHC-P||Use at an assay dependent dilution.|
ICC/IF image of ab44941 staining human prostate cells. The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C. The primary anitibody was diluted 1/400 in in 20% horse serum in PBS and incubated for 20 minutes at 4°C. An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.
The blue staining is the cell nuclei, the green staining shows the laminin beta 1.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"