Anti-Laminin beta 1抗体[LT3] (ab44941)

概述

  • 产品名称Anti-Laminin beta 1抗体[LT3]
    参阅全部 Laminin beta 1 一抗
  • 描述
    大鼠单克隆抗体[LT3] to Laminin beta 1
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Fr, IHC (Methanol fixed), IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Hamster, Human, Pig
  • 免疫原

    Murine EHS laminin preparation.

  • 阳性对照
    • LS174T cells. Normal colon or colon carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab44941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/200. Detects a band of approximately 210 kDa.
IP 1/400.
IHC-Fr 1/400. Use with acetone fixed tissues.
IHC (Methanol fixed) 1/400.
IHC-P Use at an assay dependent dilution.

靶标

  • 功能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 序列相似性Contains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • 结构域The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLM antibody
    • Cutis laxa with marfanoid phenotype antibody
    • LAM B1 antibody
    • LAMB 1 antibody
    • LAMB1 antibody
    • LAMB1_HUMAN antibody
    • Laminin B1 antibody
    • Laminin B1 chain antibody
    • Laminin beta 1 chain antibody
    • Laminin beta 1 chain precursor antibody
    • Laminin beta1 antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit beta-1 antibody
    • Laminin-1 subunit beta antibody
    • Laminin-10 subunit beta antibody
    • Laminin-12 subunit beta antibody
    • Laminin-2 subunit beta antibody
    • Laminin-6 subunit beta antibody
    • Laminin-8 subunit beta antibody
    • LIS5 antibody
    • MGC142015 antibody
    see all

Anti-Laminin beta 1 antibody [LT3] 图像

  • ICC/IF image of ab44941 staining human prostate cells.  The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C.  The primary anitibody was diluted 1/400 in in 20% horse serum in PBS and incubated for 20 minutes at 4°C.  An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.

    The blue staining is the cell nuclei, the green staining shows the laminin beta 1.

    See Abreview

  • ab44941 staining Laminin beta 1 in porcine jejunum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol/acetone, permeabilized using 0.3% Triton, blocked with 1% normal goat serum for 10 minutes at room temperature and then incubated with ab44941 at a 1/800 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rat IgG (H+L) used at a 1/200 dilution.

    See Abreview

Anti-Laminin beta 1 antibody [LT3] (ab44941)参考文献

This product has been referenced in:
  • Little GH  et al. Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion. Nucleic Acids Res 40:3538-47 (2012). WB ; Human . Read more (PubMed: 22187159) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (melanoma cell line)
Total protein in input 1000 µg
Immuno-precipitation step Protein G
Specification melanoma cell line
Username

Abcam user community

Verified customer

提交于 Nov 18 2015

Thank you for that information.

I have gone ahead and set up a credit for the cost of your original order. Once we recieve the 3 vials of unopened antibody from you the credit will be applied.

When you return the goods, please ensur...

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Thank you for your reply.

Can you please let me know how the unused units of the antibody were stored? If they have been stored at 4C since you received them, I can go ahead and credit the cost of the order back to you and also organize for t...

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Thank you for your reply and for providing that information.

I just had a few more follow up questions:

1 - You received 4 vials of ab44941. How many of the bottles did you test? I ask incase the issue may be limited to a specific b...

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Thank you for contacting Abcam.

I am sorry that you are experiencing some issues with ab44941. The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on Mouse, Hamster, Human & Pig samples . If we ca...

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Thank you for your inquiry.


When conducting IHC or IF, it is generally not advisable to use antibodies which are raised in the same species as the target sample (e.g. a mouse monoclonal antibody against mouse samples). The secondary a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (small intestine (jejunum))
Specification small intestine (jejunum)
Fixative Methanol/Acetone
Permeabilization Yes - 0.3% Triton
Blocking step (agent) for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (prostate)
Specification prostate
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton-X
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Permeabilization Yes - 0.8% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jan 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"