Anti-Laminin beta 1抗体(ab69633)

概述

  • 产品名称Anti-Laminin beta 1抗体
    参阅全部 Laminin beta 1 一抗
  • 描述
    兔多克隆抗体to Laminin beta 1
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthesized peptide derived from the C-terminus of human Laminin beta 1.

  • 阳性对照
    • Hela cells, human liver carcinoma tissue and extracts from HepG2

性能

应用

Our Abpromise guarantee covers the use of ab69633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60, 67, 110, 130, >170 kDa (predicted molecular weight: 198 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 序列相似性Contains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • 结构域The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLM antibody
    • Cutis laxa with marfanoid phenotype antibody
    • LAM B1 antibody
    • LAMB 1 antibody
    • LAMB1 antibody
    • LAMB1_HUMAN antibody
    • Laminin B1 antibody
    • Laminin B1 chain antibody
    • Laminin beta 1 chain antibody
    • Laminin beta 1 chain precursor antibody
    • Laminin beta1 antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit beta-1 antibody
    • Laminin-1 subunit beta antibody
    • Laminin-10 subunit beta antibody
    • Laminin-12 subunit beta antibody
    • Laminin-2 subunit beta antibody
    • Laminin-6 subunit beta antibody
    • Laminin-8 subunit beta antibody
    • LIS5 antibody
    • MGC142015 antibody
    see all

Anti-Laminin beta 1 antibody 图像

  • All lanes : Anti-Laminin beta 1 antibody (ab69633) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 198 kDa
    Observed band size : >170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,130 kDa,60 kDa,67 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69633 at 1:50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.
  • Immunofluorescence analysis of HeLa cells using ab69633 at a 1:500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

Anti-Laminin beta 1 antibody (ab69633)参考文献

ab69633 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab69633.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"