The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Ready to use.
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Broadly expressed in: skin, heart, lung, and the reproductive tracts.
Contains 11 laminin EGF-like domains. Contains 1 laminin IV type A domain. Contains 1 laminin N-terminal domain.
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. Domain IV is globular.
Secreted > extracellular space > extracellular matrix > basement membrane.
ab74164 staining Laminin in human skin by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note staining of basement membranes of epidermis and surrounding blood vessels.