概述

  • 产品名称Anti-Laminin抗体(Biotin)
    参阅全部 Laminin 一抗
  • 描述
    兔多克隆抗体to Laminin (Biotin)
  • 偶联物Biotin
  • 特异性Negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
  • 经测试应用适用于: ELISA, IHC-P, IP, WB, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: all Mammals
  • 免疫原

    Full length native protein (purified) corresponding to Human Laminin. Immunogen: Non-reduced 400 kDa human laminin

  • 常规说明This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use.

性能

应用

Our Abpromise guarantee covers the use of ab6571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000 - 1/100000.
IHC-P 1/1000 - 1/5000.
IP 1/100.
WB 1/2000 - 1/10000.
IHC-Fr 1/500. PubMed: 17251454

靶标

Anti-Laminin antibody (Biotin) (ab6571)参考文献

This product has been referenced in:
  • Wakuta M  et al. Delayed wound closure and phenotypic changes in corneal epithelium of the spontaneously diabetic Goto-Kakizaki rat. Invest Ophthalmol Vis Sci 48:590-6 (2007). IHC-Fr ; Rat . Read more (PubMed: 17251454) »

See 1 Publication for this product

Product Wall

We are very sorry about that. We have placed a new order one replacement vial for you - free of charge. For your information, the new order number is: 37521. We apologize for any inconvenience caused and thank you for bringing this matter to our att...

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Thank you for your enquiry. Under non reducing conditions the band of laminin should be around 900 kDa.

Unfortunately, we are not specialists in this area and we do not want to recommend a product that is unsuitable for your needs. All the of applications in which we know a product has been tested are listed on the datasheets and tested species cross rea...

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Yes, we can confirn that this antibody cross reacts with all mammalian laminins including mouse.

Sorry, we don't know the number when reduced.

This can vary greatly depending on the source of the laminin you are analysing in your blot, due to isoform variations in its' constituent alpha, beta & gamma chains.

Under non-reducing conditions, a band at approx. 400kDa is recognised. Under reducing conditions, it really depends on the source of the laminin you are analysing, due to the multiple isoforms of the alpha, beta and gamma chains.

This antibody is know to recognise most mammalian laminins, so it is expected to detect rat laminin.

This antibody is known to recognise mammalian laminin, but has not been tested for cross-reactivity with laminin from plants. I do not know of a commercially available source fo antibodies to plant laminin; however abcam has a custom antibody productio...

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This antibody reacts with the human laminin against which it was raised.It is presumed the antibody will cross-react with other mammalian laminins, because laminin is a structural protein and most structural proteins are well conserved between species.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"