概述

  • 产品名称
    Anti-Laminin抗体
    参阅全部 Laminin 一抗
  • 描述
    兔多克隆抗体to Laminin
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Mammal
  • 免疫原

    Full length native protein isolated from mouse EHS tumor(purified)

  • 阳性对照
    • This antibody gave a positive signal in MEF1 cell line in IF/ICC.

性能

应用

Our Abpromise guarantee covers the use of ab30320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 337 kDa.
ICC/IF 1/100 - 1/2500.
IHC-Fr 1/1000.
IHC-FoFr 1/1000.

靶标

  • 功能
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 组织特异性
    Broadly expressed in: skin, heart, lung, and the reproductive tracts.
  • 序列相似性
    Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • 结构域
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

图片

  • ICC/IF image of ab30320 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Rat peripheral nerve (low and high magnification; formalin fixed, frozen sections). Immuno-FITC stain (color inverted) with 1:1000 anti-Laminin1 antibody (ab30320).

文献

This product has been referenced in:
  • Jacobs K  et al. Higher-Density Culture in Human Embryonic Stem Cells Results in DNA Damage and Genome Instability. Stem Cell Reports 6:330-41 (2016). ICC/IF ; Human . Read more (PubMed: 26923824) »
  • Daubon T  et al. VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. J Cell Sci 129:2586-98 (2016). WB ; Human . Read more (PubMed: 27231093) »

See all 12 Publications for this product

客户评价及客户问答

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The antibody is a whole antiserum, without purification. As such, it is a mixture of various proteins, and immunogloblin concentration is not assayed. In general, serum is expected to contain about 10 mg/ml of to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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