Anti-Laminin抗体(ab23753)

概述

  • 产品名称Anti-Laminin抗体
    参阅全部 Laminin 一抗
  • 描述
    兔多克隆抗体to Laminin
  • 特异性Laminin fragment P1 100%, collagen type I and IV <0.1%, fibronectin <0.1%
  • 经测试应用适用于: RIA, Sandwich ELISA, ICC/IF, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified laminin P1 fragment from human placenta.

  • 阳性对照
    • Human skin
  • 常规说明


    This antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab23753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
RIA 1/1000.
Sandwich ELISA Use a concentration of 5 µg/ml. For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab49726 as Detection.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200 - 1/600.

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase in TBS 15 min at 37°C. There after, non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

ELISA Use at an assay dependent concentration.

靶标

  • 功能Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • 组织特异性Broadly expressed in: skin, heart, lung, and the reproductive tracts.
  • 序列相似性Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • 结构域The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • 细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

Anti-Laminin antibody 图像

实验方案

Anti-Laminin antibody (ab23753)参考文献

ab23753 has not yet been referenced specifically in any publications.

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This product has PBS buffer without addition of BSA and preservatives.


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