Anti-Laminin alpha 5抗体[4C7] (ab17107)

Abreviews (1)Q&A (3)Specific References (1)

概述

  • 产品名称
    Anti-Laminin alpha 5抗体[4C7]
    参阅全部 Laminin alpha 5 一抗
  • 描述
    小鼠单克隆抗体[4C7] to Laminin alpha 5
  • 宿主
    Mouse
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Full length native protein (purified) corresponding to Human Laminin alpha 5.
    Database link: O15230

  • 阳性对照
    • Skin
  • 常规说明

    This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab210957 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab17107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性
    Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.
  • 细胞定位
    Secreted; basement membrane.
  • 数据库链接
    • 别名
      • KIAA0533 antibody
      • KIAA1907 antibody
      • LAMA5 antibody
      • Laminin alpha 5 chain antibody
      • Laminin subunit alpha 5 antibody
      • Laminin subunit alpha-5 antibody
      • Laminin, alpha 5 antibody
      • Laminin-10 subunit alpha antibody
      • Laminin-11 subunit alpha antibody
      • Laminin-15 subunit alpha antibody
      • RP11-157P1.6 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5 antibody [4C7] (ab17107)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5 antibody [4C7] (ab17107)This image is courtesy of an anonymous Abreview
      ab17107 at 1/25 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. An HRP conjugated goat anti-mouse antibody was used as the secondary.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5 antibody [4C7] (ab17107)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin alpha 5 antibody [4C7] (ab17107)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal carcinoma tissue, staining Laminin alpha 5 with ab17107.

    文献

    This product has been referenced in:
    • Mackey AL  et al. Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. FASEB J 25:1943-59 (2011). IHC-Fr ; Human . Read more (PubMed: 21368102) »

    See 1 Publication for this product

    发表研究结果有使用 ab17107？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    4 reviews or Q&A

    10
    Votes: Highest First

    Dear Technical Team,

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    Please find below a complaint from one of our customerto whom we suppliedthese Abcam antibodies.

    The technical formis alsoattached in this mail.

    Request you to ki...

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    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    The antibody ab5654 is not tested in IHC-P application so Abpromise guarantee will not apply to this product. This antibody was however t...

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin antibody [4C7]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Specification
    colon
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Citric Acid Based (Vector Lab)
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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    Abcam user community

    Verified customer

    提交于 Jul 03 2007

    Hi, I don't want the detailed amino acid sequence about the epitope. Would you just please tell me the epitope locate in the middle or C terminal of laminin A? It will be suitable for my experiment if the epitope come from C terminal. Thanks again.

    Thank you for your email. Our source for this antibody has kindly provided me with the following information. In the study by Engvall, 1990, it is stated: "4C7, binds to the globular domain at the end of the long arm (Engvall et al., 1986). This domain...

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    The immunogen is stated as being "The A chain of human laminin;" is the immunogen sequence available? Or, the aa range?

    This is the only information available to us regarding the immunogen and epitope. Further details can be found in the following two publications: Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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