Anti-Laminin 5抗体[P3H9-2] (ab78286)


  • 产品名称Anti-Laminin 5抗体[P3H9-2]
    参阅全部 Laminin 5 一抗
  • 描述
    小鼠单克隆抗体[P3H9-2] to Laminin 5
  • 经测试应用适用于: WB, IP, ELISA, Inhibition Assay, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human Laminin 5. Human keratinocytes

  • 阳性对照
    • In IHC, this antibody gave a positive signal in a paraffin-embedded, formalin-fixed section of normal human colon tissue.



Our Abpromise guarantee covers the use of ab78286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 130 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.


  • 相关性Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.
  • 数据库链接

Anti-Laminin 5 antibody [P3H9-2] 图像

  • ab78286 staining Laminin 5 in Human cultured skin (NHEK, NB1RGB) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% Blocking One® for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 10% Blocking One® in PBS) for 1 hour at 25°C. An Alexa Fluor® 555-conjugated Rabbit anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • IHC image of Laminin 5 staining in human Normal Colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78286, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-Laminin 5 antibody [P3H9-2] (ab78286)参考文献

ab78286 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human skin xenograft)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Human skin xenograft
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Fixative Paraformaldehyde

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Verified customer

提交于 Sep 23 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Blocking One­ as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Tissue sections (cultured skin (NHEK, NB1RGB))
Specification cultured skin (NHEK, NB1RGB)
Permeabilization Yes - 0.1% TritonX-100
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Sep 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Tissue lysate - whole (NHEK, NB1RGB)
Specification NHEK, NB1RGB
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

提交于 Sep 23 2013

La concentration de ab78286 est de 1mg/ml, le volume unitaire est donc de 100µl.

Thank you for contacting us. For ab78286, Anti-Laminin 5 antibody [P3H9-2], Clone P3H9-2 does not specifically bind to either subunit, it should recognize all. The following literature is about the application of this clone: Oncogen(2007)26, ...

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