Anti-Laminin 5抗体[P3H9-2] (ab78286)
Key features and details
- Mouse monoclonal [P3H9-2] to Laminin 5
- Suitable for: IHC-Fr
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Laminin 5抗体[P3H9-2]
参阅全部 Laminin 5 一抗 -
描述
小鼠单克隆抗体[P3H9-2] to Laminin 5 -
宿主
Mouse -
经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-Fr: Normal human colon and skin frozen sections.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
P3H9-2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr | (2) |
Use a concentration of 1 - 5 µg/ml.
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说明 |
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IHC-Fr
Use a concentration of 1 - 5 µg/ml. |
靶标
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相关性
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. -
数据库链接
- Entrez Gene: 3909 Human
- Entrez Gene: 3914 Human
- Entrez Gene: 3918 Human
- SwissProt: Q13751 Human
- SwissProt: Q13753 Human
- SwissProt: Q16787 Human
图片
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IHC image of Laminin 5 staining in a section of frozen normal human colon*, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval step was performed prior to staining. The section was incubated with ab78286, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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IHC image of Laminin 5 staining in a section of frozen normal human skin*, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval step was performed prior to staining. The section was incubated with ab78286, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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ab78286 staining Laminin 5 in Human cultured skin (NHEK, NB1RGB) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% Blocking One® for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 10% Blocking One® in PBS) for 1 hour at 25°C. An Alexa Fluor® 555-conjugated Rabbit anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab78286 被引用在 17 文献中.
- Lee S et al. High-throughput formation and image-based analysis of basal-in mammary organoids in 384-well plates. Sci Rep 12:317 (2022). PubMed: 35013350
- Bhattacharjee S et al. PP2A phosphatase regulates cell-type specific cytoskeletal organization to drive dendrite diversity. Front Mol Neurosci 15:926567 (2022). PubMed: 36452406
- Riedl J et al. ABCB5+ dermal mesenchymal stromal cells with favorable skin homing and local immunomodulation for recessive dystrophic epidermolysis bullosa treatment. Stem Cells 39:897-903 (2021). PubMed: 33609408
- Palmer MA et al. Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle. Histochem Cell Biol 155:529-545 (2021). PubMed: 33404706
- Mestrallet G et al. Human Keratinocytes Inhibit CD4+ T-Cell Proliferation through TGFB1 Secretion and Surface Expression of HLA-G1 and PD-L1 Immune Checkpoints. Cells 10:N/A (2021). PubMed: 34201301