概述

  • 产品名称Anti-Laminin 5抗体
    参阅全部 Laminin 5 一抗
  • 描述
    兔多克隆抗体to Laminin 5
  • 经测试应用适用于: IP, ICC/IF, IHC-Fr, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Horse, Dog, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Laminin 5.

性能

应用

Our Abpromise guarantee covers the use of ab14509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 16940506
ICC/IF Use at an assay dependent concentration. PubMed: 16940506
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 165, 140, 105 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.
  • 数据库链接

Anti-Laminin 5 antibody 图像

  • ICC/IF image of ab14509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Laminin 5 antibody (ab14509)参考文献

This product has been referenced in:
  • Brock A  et al. Silencing HoxA1 by intraductal injection of siRNA lipidoid nanoparticles prevents mammary tumor progression in mice. Sci Transl Med 6:217ra2 (2014). Mouse, Human . Read more (PubMed: 24382894) »
  • Montagner A  et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med 6:80-98 (2014). IF . Read more (PubMed: 24203162) »

See all 21 Publications for this product

Product Wall

Thank you for your enquiry and my apologies for the delay in replying to you. The antibody ab14509 is not produced in Abcam's laboratories but comes from a supplier and therefore we do not have details regarding a protocol for protein G purification...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Uterus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Heat Mediate Buffer/Enzyme used (Citrate TBE)
Permeabilization No
Specification Uterus
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Ms. Devin Columbus

Verified customer

提交于 Sep 12 2015

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Merci de nous avoir contactés.

Les FDS (ou MSDS) sont disponiblesdirectement depuis les fiches techniques de nos produits:
http://www.abcam.com/ab83774
http://www.abcam.com/ab14509

Comme expliqué dans mes pr&eacu...

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Thank you for contacting us and for your interest in our products.

I am sorry to confirm that as far as we are aware, none of our laminin 5 antibodies has been tested on pig tissue. All tested species cross-reactivity and tested applications...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HaCaT)
Loading amount 40 µg
Specification HaCaT
Gel Running Conditions Reduced Denaturing (7%, 6 V, 60 min.)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Mar 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast)
Loading amount 10 µg
Specification Breast
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Yoon Pyo Choi

Verified customer

提交于 Jun 25 2009

Thank you for your enquiry. I can recommend using a starting dilution of 1ug/ml for WB. However, please bear in mind that optimum conditions/concentrations should be determined by the end user. I hope this will help you get started with your ex...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (grafted human skin)
Specification grafted human skin
Fixative Acetone
Blocking step 10% normal goat serum + 0.1% BSA in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Dr. Cynthia Simbulan-Rosenthal

Verified customer

提交于 Jun 28 2007

Thank you for your enquiry. Laminin 5 dissociates into 3 fragments in Western Blot: 1. alpha 3 chain - 165 kDa 2. beta 3 chain - 140 kDa 3. gamma 2 chain - 105 kDa I hope this information helps, please do not hesitate to contact us if you need...

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1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"