概述

  • 产品名称Anti-Lamin B2抗体
    参阅全部 Lamin B2 一抗
  • 描述
    兔多克隆抗体to Lamin B2
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Xenopus laevis
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 221-470 of Human Lamin B2 (NP_116126)

  • 阳性对照
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells IHC-P: Cal27 xenograft

性能

应用

Our Abpromise guarantee covers the use of ab97513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22341447
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/250.

靶标

  • 功能Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • 疾病相关Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • 细胞定位Nucleus inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LAMB 2 antibody
    • LAMB2 antibody
    • Lamin-B2 antibody
    • LMN 2 antibody
    • LMN B2 antibody
    • LMN2 antibody
    • LMNB 2 antibody
    • LMNB2 antibody
    • LMNB2_HUMAN antibody
    • MGC2721 antibody
    • RGD1563803 antibody
    see all

Anti-Lamin B2 antibody 图像

  • All lanes : Anti-Lamin B2 antibody (ab97513) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 68 kDa
  • Immunohistochemical analysis of Lamin B2 in paraffin embedded Cal27 Xenograft, using ab97513 at a 1/100 dilution.

Anti-Lamin B2 antibody (ab97513)参考文献

This product has been referenced in:
  • Yoon BC  et al. Local translation of extranuclear lamin B promotes axon maintenance. Cell 148:752-64 (2012). ICC/IF ; Xenopus laevis . Read more (PubMed: 22341447) »

See 1 Publication for this product

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