概述

  • 产品名称Anti-Lamin B1抗体[ZL-5]
    参阅全部 Lamin B1 一抗
  • 描述
    小鼠单克隆抗体[ZL-5] to Lamin B1
  • 特异性The antibody recognizes the 68 kDa lamin B1 isoform. Reactivity with other lamin isoforms has not been detected.
  • 经测试应用适用于: Flow Cyt, ICC/IF, WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    Recombinant human Lamin B.

性能

应用

Our Abpromise guarantee covers the use of ab20396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/20. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18798337
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 68 kDa.
IP Use a concentration of 5 µg/ml.
ELISA Use a concentration of 0.2 - 1 µg/ml.

靶标

  • 功能Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • 疾病相关Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • 细胞定位Nucleus inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Anti-Lamin B1 antibody [ZL-5] 图像

  • Anti-Lamin B1 antibody [ZL-5] (ab20396) at 0.2 µg/ml + HeLa cell lysates
  • Overlay histogram showing HeLa cells stained with ab20396 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20396, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave no signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Lamin B1 antibody [ZL-5] (ab20396)参考文献

This product has been referenced in:
  • Furfaro AL  et al. HO-1 up-regulation: a key point in high-risk neuroblastoma resistance to bortezomib. Biochim Biophys Acta 1842:613-22 (2014). WB . Read more (PubMed: 24380881) »
  • Snodgrass SM  et al. Tgf-ß1 inhibits Cftr biogenesis and prevents functional rescue of ?F508-Cftr in primary differentiated human bronchial epithelial cells. PLoS One 8:e63167 (2013). WB ; Human . Read more (PubMed: 23671668) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - nuclear (Mouse Gatsrocnemius)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 50 µg
Specification Mouse Gatsrocnemius
Blocking step Seablock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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Verified customer

提交于 Nov 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa)
Total protein in input 1000 µg
Specification HeLa
Immuno-precipitation step Other - Dynabeads protein G
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提交于 Jul 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (beta-cells line)
Loading amount 25 µg
Specification beta-cells line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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提交于 May 14 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"