The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 18798337
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 68 kDa.
Use a concentration of 5 µg/ml.
Use a concentration of 0.2 - 1 µg/ml.
功能Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
疾病相关Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
序列相似性Belongs to the intermediate filament family.
翻译后修饰B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Western blot - Anti-Lamin B1 antibody [ZL-5] (ab20396)
Anti-Lamin B1 antibody [ZL-5] (ab20396) at 0.2 µg/ml + HeLa cell lysates
Flow Cytometry-Lamin B1 antibody [ZL-5](ab20396)
Overlay histogram showing HeLa cells stained with ab20396 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20396, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave no signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Anti-Lamin B1 antibody [ZL-5] (ab20396)参考文献
This product has been referenced in:
Furfaro AL et al. HO-1 up-regulation: a key point in high-risk neuroblastoma resistance to bortezomib. Biochim Biophys Acta1842:613-22 (2014).
Read more (PubMed: 24380881) »
Snodgrass SM et al. Tgf-ß1 inhibits Cftr biogenesis and prevents functional rescue of ?F508-Cftr in primary differentiated human bronchial epithelial cells. PLoS One8:e63167 (2013).
Read more (PubMed: 23671668) »