The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 66 kDa).
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
Belongs to the intermediate filament family.
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Lane 1: Wild type HAP1 whole cell lysate (20 µg) Lane 2: Empty Lane 3: LMNB1 HAP1 knockout whole cell lysate (20 µg) Lane 4: Empty Lanes 1 - 4: Merged signal (red and green). Green - ab90169 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab90169 was shown to specifically react with Lamin B1 in wild-type HAP1 cells. No bands were observed when Lamin B1 knockout samples were used. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. ab90169 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Donkey anti-Chicken IgG H&L (IRDye® 800CW) and Donkey anti Mouse IgG H&L secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.