Anti-Lamin B1抗体(ab65986)
Key features and details
- Rabbit polyclonal to Lamin B1
- Suitable for: IHC-Fr, WB, IHC-P, ICC
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Lamin B1抗体
参阅全部 Lamin B1 一抗 -
描述
兔多克隆抗体to Lamin B1 -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, WB, IHC-P, ICCmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Lamin B1 aa 570-586 (C terminal).
Sequence:FHQQGTPRASNRSCAIM
Database link: P20700 -
阳性对照
- WB: Rat brain tissue lysate IHC-P:Mammary cancer sections; IHC-F: Rat Intestine Tissue ICC: A549 Cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65986于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml. Formalin or acetone fixed tissues.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC |
Use a concentration of 0.5 - 1 µg/ml. Acetone fixed.
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说明 |
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IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. Formalin or acetone fixed tissues. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa). |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC
Use a concentration of 0.5 - 1 µg/ml. Acetone fixed. |
靶标
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功能
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. -
疾病相关
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. -
细胞定位
Nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4001 Human
- Entrez Gene: 116685 Rat
- Omim: 150340 Human
- SwissProt: P20700 Human
- SwissProt: P70615 Rat
- Unigene: 89497 Human
- Unigene: 11362 Rat
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别名
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
see all
图片
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All lanes : Anti-Lamin B1 antibody (ab65986)
Lane 1 : MCF-7 Cell lysate
Lane 2 : HeLa Cell lysate
Predicted band size: 66 kDa -
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Immunohistochemistry (Frozen sections) of Rat Intestine Tissue staining Lamin B1 with ab65986
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Immunocytochemistry of A549 Cells staining Lamin B1 with ab65986.
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Anti-Lamin B1 antibody (ab65986) at 1 µg/ml + rat brain tissue lysate
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab65986 被引用在 39 文献中.
- Ke B et al. APC ameliorates idiopathic membranous nephropathy by affecting podocyte apoptosis through the ERK1/2/YB-1/PLA2R1 axis. Mol Cell Biochem N/A:N/A (2023). PubMed: 36588134
- Lotlikar MS et al. Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix. STAR Protoc 3:101028 (2022). PubMed: 35059649
- Ni H et al. Cardamonin attenuates cerebral ischemia/reperfusion injury by activating the HIF-1α/VEGFA pathway. Phytother Res 36:1736-1747 (2022). PubMed: 35142404
- Lee SH et al. Povidone iodine suppresses LPS-induced inflammation by inhibiting TLR4/MyD88 formation in airway epithelial cells. Sci Rep 12:3681 (2022). PubMed: 35256715
- Rivera C et al. Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin. Nat Commun 13:1550 (2022). PubMed: 35322029