Anti-Lamin B1抗体[119D5-F1] (ab8982)

概述

  • 产品名称Anti-Lamin B1抗体[119D5-F1]
    参阅全部 Lamin B1 一抗
  • 描述
    小鼠单克隆抗体[119D5-F1] to Lamin B1
  • 特异性Reacts with an epitope located C-terminal of residue 231 in lamin B1. Reacts against lamin B1, does not cross react with lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. ab8982 has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested.
  • 经测试应用适用于: WB, Dot blot, ELISA, Flow Cyt, ICC, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Zebrafish
  • 免疫原

    Tissue, cells or virus corresponding to Rat Lamin B1 (C terminal). Immunogen = Purified Rat liver lamins

  • 表位C-terminal to residue 231.
  • 阳性对照
    • 3T3 cells

性能

应用

Our Abpromise guarantee covers the use of ab8982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/1000. Predicted molecular weight: 67 kDa.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC 1/20 - 1/50.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • 疾病相关Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • 序列相似性Belongs to the intermediate filament family.
  • 翻译后修饰B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • 细胞定位Nucleus inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Anti-Lamin B1 antibody [119D5-F1] 图像

  • Methanol fixed HeLa and 3T3 cells (ab7179) were stained with ab8982 (1/50 and 1/20 respectively). The cells were fixed in 100% methanol for 6 minutes at -20°C. ab8982 clearly stains the nuclear envelope with very little backgrouns staining. 

    A: HeLa cells + ab8982 (1/50) (green)
    B: HeLa cells counterstained with DAPI (blue)
    C. 3T3 cells + ab8982 (1/20) (green)
    D. 3T3 cells counterstained with DAPI (blue)

  • All lanes : Anti-Lamin B1 antibody [119D5-F1] (ab8982) at 1/2000 dilution

    Lane 1 : Whole cell lysate prepared from human mammary cells (24 hour culture)
    Lane 2 : Whole cell lysate prepared from human mammary cells (48 hour culture)

    Lysates/proteins at 50000 cells per lane.

    Secondary
    HRP conjugated goat anti-mouse polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 67 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 40 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Immunofluorescence staining images of 9 day old zebrafish embryos. Frozen sections fixed in Acetone:Methanol 1:1 ab8982 diluted 1/100, incubated for 45 minutes at room temperature.
  • Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells
  • Immunohistochemistry on frozen sections of swine liver showing nuclear lamina staining in hepatocytes
  • Overlay histogram showing HeLA cells stained with ab8982 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8982, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Lamin B1 antibody [119D5-F1] (ab8982)参考文献

This product has been referenced in:
  • Lu Q  et al. Ca²?/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures. PLoS One 8:e70750 (2013). WB ; Rat . Read more (PubMed: 23976956) »
  • Joseph S  et al. ARID3B induces tumor necrosis factor alpha mediated apoptosis while a novel ARID3B splice form does not induce cell death. PLoS One 7:e42159 (2012). Read more (PubMed: 22860069) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Non-reduced Denaturing (8%)
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step 5% BSA + 5% Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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提交于 Oct 27 2014

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Thank you for your enquiry regarding ab8982 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human mammary cell)
Loading amount 50000 cells
Specification Human mammary cell
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon cell line)
Loading amount 20 µg
Specification Colon cell line
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 30 2010

I'm sorry to hear you are having a problem with ab8982. This antibody has been purchased many times in the past few months without any reported problems, so I am fairly confident that we can modify your protocol and get the antibody to work for you....

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We have received information from the supplier of ab8982 apologising for this problem and currently re-testing the antibody and offering a replacement vial as soon as the new batch is quality approved. I was unfortunately unable to find your order ...

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