重组
RabMAb

Anti-Lamin B Receptor抗体[E398L] (ab32535)

概述

性能

应用

Our Abpromise guarantee covers the use of ab32535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/50.
WB 1/500. Detects a band of approximately 67 kDa (predicted molecular weight: 71 kDa).
ICC/IF 1/500.
Flow Cyt Use at an assay dependent concentration.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Anchors the lamina and the heterochromatin to the inner nuclear membrane.
  • 疾病相关
    Defects in LBR are a cause of Pelger-Huet anomaly (PHA) [MIM:169400]. PHA is an autosomal dominant inherited abnormality of neutrophils, characterized by reduced nuclear segmentation and an apparently looser chromatin structure. Heterozygotes show hypolobulated neutrophil nuclei with coarse chromatin. Presumed homozygous individuals have ovoid neutrophil nuclei, as well as varying degrees of developmental delay, epilepsy, and skeletal abnormalities.
    Defects in LBR are the cause of hydrops-ectopic calcification-moth-eaten skeletal dysplasia (HEM) [MIM:215140]; also known as Greenberg skeletal dysplasia. HEM is a rare autosomal recessive chondrodystrophy characterized by early in utero lethality and, therefore, considered to be nonviable. Affected fetuses typically present with fetal hydrops, short-limbed dwarfism, and a marked disorganization of chondro-osseous calcification and may present with polydactyly and additional nonskeletal malformations.
    Defects in LBR may be a cause of Reynolds syndrome (REYNS) [MIM:613471]. It is a syndrome specifically associating limited cutaneous systemic sclerosis and primary biliray cirrhosis. It is characterized by liver disease, telangiectasia, abrupt onset of digital paleness or cyanosis in response to cold exposure or stress (Raynaud phenomenon), and variable features of scleroderma. The liver disease is characterized by pruritis, jaundice, hepatomegaly, increased serum alkaline phosphatase and positive serum mitochondrial autoantibodies, all consistent with primary biliary cirrhosis.
  • 序列相似性
    Belongs to the ERG4/ERG24 family.
  • 翻译后修饰
    Phosphorylated by CDK1 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.
  • 细胞定位
    Nucleus inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DHCR 14B antibody
    • DHCR14B antibody
    • Integral nuclear envelope inner membrane protein antibody
    • Lamin-B receptor antibody
    • LBR antibody
    • LBR_HUMAN antibody
    • LMN 2R antibody
    • LMN2R antibody
    • MGC9041 antibody
    • PHA antibody
    • PRO0650 antibody
    see all

Anti-Lamin B Receptor antibody [E398L] 图像

  • Ab32535, at a 1/500 dilution, staining Lamin B Receptor in HeLa cells by Immunofluorescence.
  • Anti-Lamin B Receptor antibody [E398L] (ab32535) at 1/500 dilution + Jurkat cell lysate

    Predicted band size : 71 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Lamin B Receptor with unpurified ab32535 at 1/20 dilutionc(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • IHC image of ab32535 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32535, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Lamin B Receptor antibody [E398L] (ab32535)参考文献

This product has been referenced in:
  • Cundell MJ  et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol 214:539-54 (2016). Read more (PubMed: 27551054) »
  • Mimura Y  et al. ELYS regulates the localization of LBR by modulating its phosphorylation state. J Cell Sci 129:4200-4212 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27802161) »

See all 10 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse embryonic fibroblasts)
Permeabilization
Yes - 0.5% triton X
Specification
Mouse embryonic fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 12 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (NCI-H929)
Loading amount
200000 cells
Specification
NCI-H929
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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