Anti-Lamin B Receptor抗体[E398L] (ab32535)




Our Abpromise guarantee covers the use of ab32535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/50.
WB 1/500. Detects a band of approximately 67 kDa (predicted molecular weight: 71 kDa).
ICC/IF 1/500.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Anchors the lamina and the heterochromatin to the inner nuclear membrane.
    • 疾病相关Defects in LBR are a cause of Pelger-Huet anomaly (PHA) [MIM:169400]. PHA is an autosomal dominant inherited abnormality of neutrophils, characterized by reduced nuclear segmentation and an apparently looser chromatin structure. Heterozygotes show hypolobulated neutrophil nuclei with coarse chromatin. Presumed homozygous individuals have ovoid neutrophil nuclei, as well as varying degrees of developmental delay, epilepsy, and skeletal abnormalities.
      Defects in LBR are the cause of hydrops-ectopic calcification-moth-eaten skeletal dysplasia (HEM) [MIM:215140]; also known as Greenberg skeletal dysplasia. HEM is a rare autosomal recessive chondrodystrophy characterized by early in utero lethality and, therefore, considered to be nonviable. Affected fetuses typically present with fetal hydrops, short-limbed dwarfism, and a marked disorganization of chondro-osseous calcification and may present with polydactyly and additional nonskeletal malformations.
      Defects in LBR may be a cause of Reynolds syndrome (REYNS) [MIM:613471]. It is a syndrome specifically associating limited cutaneous systemic sclerosis and primary biliray cirrhosis. It is characterized by liver disease, telangiectasia, abrupt onset of digital paleness or cyanosis in response to cold exposure or stress (Raynaud phenomenon), and variable features of scleroderma. The liver disease is characterized by pruritis, jaundice, hepatomegaly, increased serum alkaline phosphatase and positive serum mitochondrial autoantibodies, all consistent with primary biliary cirrhosis.
    • 序列相似性Belongs to the ERG4/ERG24 family.
    • 翻译后修饰Phosphorylated by CDK1 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.
    • 细胞定位Nucleus inner membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DHCR 14B antibody
      • DHCR14B antibody
      • Integral nuclear envelope inner membrane protein antibody
      • Lamin-B receptor antibody
      • LBR antibody
      • LBR_HUMAN antibody
      • LMN 2R antibody
      • LMN2R antibody
      • MGC9041 antibody
      • PHA antibody
      • PRO0650 antibody
      see all

    Anti-Lamin B Receptor antibody [E398L] 图像

    • Anti-Lamin B Receptor antibody [E398L] (ab32535) at 1/500 dilution + Jurkat cell lysate

      Predicted band size : 71 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    • Ab32535, at a 1/500 dilution, staining Lamin B Receptor in HeLa cells by Immunofluorescence.
    • IHC image of ab32535 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32535, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-Lamin B Receptor antibody [E398L] (ab32535)参考文献

    This product has been referenced in:
    • Cundell MJ  et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol 214:539-54 (2016). Read more (PubMed: 27551054) »
    • Wang Y  et al. p32 is a novel target for viral protein ICP34.5 of herpes simplex virus type 1 and facilitates viral nuclear egress. J Biol Chem 289:35795-805 (2014). Read more (PubMed: 25355318) »

    See all 9 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (NCI-H929)
    Loading amount 200000 cells
    Specification NCI-H929
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

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    提交于 Apr 20 2012