重组Anti-Lamin A + Lamin B1 + Lamin C抗体[EPR4068] (ab108922)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4068] to Lamin A + Lamin B1 + Lamin C
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Lamin A + Lamin B1 + Lamin C抗体[EPR4068]
参阅全部 Lamin A+Lamin B1+Lamin C 一抗 -
描述
兔单克隆抗体[EPR4068] to Lamin A + Lamin B1 + Lamin C -
宿主
Rabbit -
特异性
The antibody recognizes full length Lamin A/B1/C and the cleaved small unit.
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经测试应用
适用于: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Spermophilus tridecemlineatus -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse heart and rat heart tissue lysates and HeLa, HepG2 and HACAT cell lysates, ab83472. IHC-P: Human ovarian carcinoma, human liver carcinoma, human breast, human uterus, human kidney mouse liver and rat liver tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HepG2 lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4068 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223942)
- Alexa Fluor® 647 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab223943)
- Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] - BSA and Azide free (ab226043)
- Alexa Fluor® 568 Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab312420)
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108922于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/20.
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WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/20. |
WB
1/1000 - 1/10000. Predicted molecular weight: 74 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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细胞定位
Lamin A: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C. Lamin B1: Nucleus inner membrane. Lamin C: Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C. -
数据库链接
- Entrez Gene: 4000 Human
- Entrez Gene: 4001 Human
- Entrez Gene: 16905 Mouse
- Entrez Gene: 16906 Mouse
- Entrez Gene: 116685 Rat
- Entrez Gene: 60374 Rat
- Omim: 150330 Human
- Omim: 150340 Human
see all -
形式
Lamin C: There are 3 isoforms produced by alternative splicing. Isoform A also known as Lamin A; Isoform C also known as Lamin C; Isoform ADelta10 also known as Lamin ADelta10.
图片
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All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/10000 dilution (purified)
Lane 1 : mouse heart lysate
Lane 2 : rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution
Predicted band size: 74 kDa
Additional bands at: 65 kDa (possible isoform), 70 kDa (possible isoform), 74 kDa (possible isoform)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunofluorescence staining of HeLa cells with purified ab108922 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108922 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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Immunohistochemical staining of paraffin embedded rat liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution
Lane 1 :Recombinant Human Lamin A protein (ab83472)
Lane 2 : GST tagged recombinant Human Lamin B1 protein (1 to 586) (91 KDa)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 75, 91 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
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Lane 1 : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution
Lane 2 : Anti-Myc tag antibody at 1/10000 dilution
Lane 3 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/5000 dilution
All lanes : Myc and DDK tagged recombinant Human Lamin B2 protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
ab108922 does not cross react with Lamin B2.
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Intracellular Flow Cytometry analysis of HeLa cells labelling Lamin A + B1 + C with purified ab108922 at a dilution of 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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ab108922 (purified) at 1/20 immunoprecipitating Lamin A + B1 + C in 10 μg HepG2 (Lanes 1 and 2, observed at 70, 74, and 65 kDa - ab108922 recognises three isoforms). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Dilution buffer and concentration: 5% NFDM/TBST.
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Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution
Predicted band size: 74 kDa
Additional bands at: 65 kDa (possible isoform), 70 kDa (possible isoform), 74 kDa (possible isoform)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded human liver carcinoma with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded mouse liver with purified ab108922 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical analysis of paraffin-embedded human colonic tissue using unpurified ab108922 at a diltion of 1/100
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All lanes : Anti-Lamin A + Lamin B1 + Lamin C antibody [EPR4068] (ab108922) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 74 kDa -
Unpurified ab108922 showing positive staining in Normal human breast tissue.
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Unpurified ab108922 showing positive staining in Normal human uterus tissue.
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Unpurified ab108922 showing positive staining in Normal human kidney tissue.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (29)
ab108922 被引用在 29 文献中.
- Zhou C & Homer HA The oocyte spindle midzone pauses Cdk1 inactivation during fertilization to enable male pronuclear formation and embryo development. Cell Rep 39:110789 (2022). PubMed: 35508138
- Knox J et al. MiR-18a-5p Targets Connective Tissue Growth Factor Expression and Inhibits Transforming Growth Factor β2-Induced Trabecular Meshwork Cell Contractility. Genes (Basel) 13:N/A (2022). PubMed: 36011411
- Sui H et al. Manganese enhances DNA- or RNA-mediated innate immune response by inducing phosphorylation of TANK-binding kinase 1. iScience 25:105352 (2022). PubMed: 36325059
- Sharma BK et al. Fibrinogen activates focal adhesion kinase (FAK) promoting colorectal adenocarcinoma growth. J Thromb Haemost 19:2480-2494 (2021). PubMed: 34192410
- Fan G et al. The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation. Nat Commun 12:20 (2021). PubMed: 33397932