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Recombinant fragment corresponding to Human Lamin A (C terminal). Around aa 598 to 611.
Our Abpromise guarantee covers the use of ab8980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100 - 1/200. ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.|
|ICC||1/100 - 1/200.|
|IHC-Fr||1/100 - 1/200.|
|WB||1/100 - 1/1000. Predicted molecular weight: 74 kDa. PubMed: 92745311/1000. Predicted molecular weight: 74 kDa.|
|IHC-P||1/100 - 1/200.|
|ICC/IF||Use a concentration of 1 µg/ml.|
ICC/IF image of ab8980 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8980 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at room temperature
ab8980 staining of Lamin A in adult mouse liver sections. Formaldehyde-fixed paraffin-embedded sections of mouse liver tissue were incubated with ab8980 (1/2500) fo 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0 (ab64214). Please see accompanying abreview for additional information.
Immunohistochemistry on paraffin section of human colon.
Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.