Anti-Lambda Free Light Chain抗体[3D12] (ab1944)


  • 产品名称Anti-Lambda Free Light Chain抗体[3D12]
    参阅全部 Lambda Free Light Chain 一抗
  • 描述
    小鼠单克隆抗体[3D12] to Lambda Free Light Chain
  • 特异性Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.
  • 经测试应用适用于: WB, ELISA, Other, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Mixture of light chains of human immunoglobulin.

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
  • 常规说明Concentration varies from lot to lot and can be provided on request.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purity tested by electrophoresis.
  • 克隆单克隆
  • 克隆编号3D12
  • 骨髓瘤Sp2/0
  • 同种型IgG2a
  • 研究领域


Our Abpromise guarantee covers the use of ab1944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.


  • 相关性standard

Anti-Lambda Free Light Chain antibody [3D12] 图像

  • IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Lambda Free Light Chain antibody [3D12] (ab1944)参考文献

ab1944 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (blood cells)
Permeabilization No
Gating Strategy Lymphoid gate only
Specification blood cells
Preparation Cell harvesting/tissue preparation method: hypotonic lysis of erythrocytes
Sample buffer: phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin from Cold Water Fish Skin
Fixation None

Dr. Marek Sinkora

Verified customer

提交于 Jun 20 2016

Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry.

The protocolused for Western blotting was according to Towbin et al. in: PNAS, September 1, 1979, vol. 76, no. 9, 4350-435...

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