概述

  • 产品名称Anti-LAIR1抗体[NKTA255] (FITC)
    参阅全部 LAIR1 一抗
  • 描述
    小鼠单克隆抗体[NKTA255] to LAIR1 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    NK cells lines B12.100 and AM.25 (Human)

  • 常规说明


    Monoclonal antibodies of clone NKTA255, such as ab27744, are reported to inhibit NK cell triggering via CD16 molecules.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明Monoclonal antibodies of clone NKTA255, such as ab27744, are reported to inhibit NK cell triggering via CD16 molecules.
  • 克隆单克隆
  • 克隆编号NKTA255
  • 骨髓瘤P3U1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab27744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells.

10µl of neat antibody for 106 cells in 100µl.





ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
  • 组织特异性Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
  • 序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 发展阶段Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
  • 结构域ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
  • 翻译后修饰Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
    N-glycosylated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5133400O11Rik antibody
    • CD305 antibody
    • D7Bwg0421e antibody
    • hLAIR1 antibody
    • LAIR 1 antibody
    • LAIR-1 antibody
    • LAIR1 antibody
    • LAIR1 protein antibody
    • LAIR1_HUMAN antibody
    • Leukocyte associated Ig like receptor 1 antibody
    • Leukocyte associated immunoglobulin like receptor 1 antibody
    • Leukocyte-associated immunoglobulin-like receptor 1 antibody
    see all

Anti-LAIR1 antibody [NKTA255] (FITC) 图像

  • Flow Cytometry analysis of human peripheral blood lymphocytes labeling LAIR1 with FITC-conjugated ab27744.

Anti-LAIR1 antibody [NKTA255] (FITC) (ab27744)参考文献

This product has been referenced in:
  • Exley M  et al. CD161 (NKR-P1A) costimulation of CD1d-dependent activation of human T cells expressing invariant V alpha 24 J alpha Q T cell receptor alpha chains. J Exp Med 188:867-76 (1998). Read more (PubMed: 9730888) »
  • Poggi A  et al. p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans. Eur J Immunol 25:369-76 (1995). Read more (PubMed: 7875198) »

See all 2 Publications for this product

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