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In Abcam's Lactose Assay Kit Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (OD570nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.
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Lactose (C12H22O11 FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.
|HRP (Lyophilized)||Purple||1 vial|
|Lactase (Lyophilized)||Blue||1 vial|
|Lactose Assay Buffer||WM||1 x 25ml|
|Lactose Enzyme Mix (Lyophilized)||Green||1 vial|
|Lactose Standard (100 nmol/ul)||Yellow||1 x 100µl|
|Probe (DMSO solution)||Red||1 x 200µl|
Our Abpromise guarantee covers the use of ab83384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|