概述

  • 产品名称Lactose Assay试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Other biological fluids, Tissue Extracts
  • 检测类型Quantitative
  • 检测时间
    1h 00m
  • 产品概述

    In Abcam's Lactose Assay Kit Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (OD570nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Lactose (C12H22O11 FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Lactose (C12H22O11 FW: 342.3) is an important naturally occurring disaccharide along with sucrose and maltose. Lactose makes up around 2-8% of milk. It consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.
  • 别名
    • Milk sugar

应用

Our Abpromise guarantee covers the use of ab83384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Lactose Assay Kit 图像

  • Lactose Standard Curve: Assays were performed following the kit instructions. The kit detected galactose and lactose equally. In the absence of Lactase, the kit detected galactose, but not lactose.

实验方案

Lactose Assay Kit (ab83384)参考文献

This product has been referenced in:
  • Dempsey C  et al. Marginal maternal zinc deficiency in lactating mice reduces secretory capacity and alters milk composition. J Nutr 142:655-60 (2012). Mouse . Read more (PubMed: 22357740) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry and I apologize for the delay in this reply as I was in touch with the testing laboratory.

Are youdoing a colorimetric or a fluorometric assay? I'm a little confused because your data says (544, 590), but this isn't...

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Thank you very much for your call today, and I apologize again for the mistake in our protocol.
My colleagues will update the protocol shortly, but here is the omitted step for your reference:
"3. Add 2 uL of Lactase into each standard and sa...

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Thank you for your inquiry.

I can confirm that dairy products can be used with ab83384. Since they have not beenspecifically tested, we strongly suggest testing several doses to make sure the readings are within the standard curve linear rang...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"