Anti-Lactoferrin抗体(ab77705)

概述

  • 产品名称Anti-Lactoferrin抗体
    参阅全部 Lactoferrin 一抗
  • 描述
    兔多克隆抗体to Lactoferrin
  • 经测试应用适用于: IHC-P, WB, IP, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Full length native mouse Lactoferrin

  • 阳性对照
    • Mouse uterine fluid. Female rat reproductive tract sections. FFPE human diabetic bone marrow tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab77705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/10000 - 1/20000. Predicted molecular weight: 79 kDa.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

靶标

  • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • 序列相似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Anti-Lactoferrin antibody 图像

  • Anti-Lactoferrin antibody (ab77705) at 1/20000 dilution + Mouse uterine fluid

    Secondary
    Goat anti-rabbit HRP conjugate
    Developed using the ECL technique

    Predicted band size : 79 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • IHC image of Lactofferin staining in human diabetic bone marrow formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77705, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Lactoferrin antibody (ab77705)参考文献

ab77705 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

Unfortunately, as I was mentioning in my previous email, the antibodies we have tested in mouse were raised against the full length protein. Thus, we do not know the binding sites and such the potential cross-reactivi...

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Thank you for your reply.

I was wondering how did you confirm that the lactoferrin antibody crossreacts with transferrin? The MW of both mouse proteins (serotransferrin in particular) are almost identical (78 vs 77 kDa). These cannot be distin...

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Thank youand your customer very much for your interest in ab77705.

ab77705 has been tested for WB, IP and ICC so far.

To our knowledge,ab77705 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if...

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If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know which antibody you would like to test (Ab77780 or Ab38155). I will then send a discount code. This code must be issued before purchasing the antibody...

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Thanks for your call yesterday and for your patience while I have looked through our lactoferrin antibodies. Ab77780 would be a good antibody to try, as it will probably cross-react with mouse, though I can't say that it won't react with transferrin as...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"