• 产品名称Anti-Lactoferrin抗体
    参阅全部 Lactoferrin 一抗
  • 描述
    山羊多克隆抗体to Lactoferrin
  • 经测试应用适用于: ELISA, WB, Sandwich ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide:


    , corresponding to internal sequence amino acids 432-444 of Human Lactoferrin (NP_002334.2).

  • 阳性对照
    • Human Peripheral Blood Mononucleocyte lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab77548 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab77548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 85 kDa.
Sandwich ELISA Use a concentration of 5 - 10 µg/ml.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • 序列相似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Anti-Lactoferrin antibody 图像

  • Anti-Lactoferrin antibody (ab77548) at 0.05 µg/ml + Human Peripheral Blood Mononucleocyte lysate (in RIPA buffer) at 35 µg

    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • ab77548 (2µg/ml) staining of paraffin embedded Human Blood Vessel shows strong staining of cytoplasm of neutrophils. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. ab77548 reporter concentration: 5 ug/ml
  • Anti-Lactoferrin antibody (ab77548) at 0.2 µg/ml + Human Lactoferrin full length protein (ab78526) at 0.01 µg/ml

    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

Anti-Lactoferrin antibody (ab77548)参考文献

ab77548 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77548.
Please use the links above to contact us or submit feedback about this product.