概述

  • 产品名称Anti-Lactoferrin抗体
    参阅全部 Lactoferrin 一抗
  • 描述
    兔多克隆抗体to Lactoferrin
  • 经测试应用适用于: IHC-P, ELISA, WB, IP, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human).

  • 常规说明


    Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Antibody stabilization buffer.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity purified using immobilized antigenic proteins and pre adsorbed on an immobilized heterologus lactoferin matrix to remove the cross reacting antibodies.
  • Primary antibody说明Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
ELISA
WB
IP
ICC
  • 应用说明ELISA: Use at an assay dependent dilution (PMID 17244155).
    ICC: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IP: 1/200.
    WB: 1/500. Predicted molecular weight: 79 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
      Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
      Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
      The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
    • 序列相似性Belongs to the transferrin family.
      Contains 2 transferrin-like domains.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GIG12 antibody
      • Growth inhibiting protein 12 antibody
      • HLF2 antibody
      • Kaliocin 1 antibody
      • Lactoferrin antibody
      • Lactoferroxin-C antibody
      • Lactotransferrin antibody
      • LF antibody
      • LTF antibody
      • Neutrophil lactoferrin antibody
      • Talalactoferrin antibody
      • TRFL_HUMAN antibody
      see all

    Anti-Lactoferrin antibody 图像

    • Anti-Lactoferrin antibody (ab15811) at 1/500 dilution + Human Lactoferrin

      Predicted band size : 79 kDa
      Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    • ab15811 at a 1/150 dilution staining Lactoferrin in human tonsil and intestinal tissue sections by Immunohistochemistry (paraffin embedded) incubated for 20 minutes at 25°C. Fixed with formaldehyde, enzymatic antigen retrieval step performed using proteinase K for 5 minutes at 37°C. Secondary used undiluted polyclonal Goat anti-mouse/rabbit IgG.

      See Abreview

    Anti-Lactoferrin antibody (ab15811)参考文献

    This product has been referenced in:
    • Zonneveld MI  et al. Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures. J Extracell Vesicles 3:N/A (2014). WB ; Human . Read more (PubMed: 25206958) »
    • Rousseau E  et al. The iron-binding protein lactoferrin protects vulnerable dopamine neurons from degeneration by preserving mitochondrial calcium homeostasis. Mol Pharmacol 84:888-98 (2013). Human . Read more (PubMed: 24077968) »

    See all 8 Publications for this product

    Product Wall

    Application ELISA
    Sample Human Cell (stool)
    Specification stool
    Type Sandwich (Capture)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 22°C
    Username

    Mrs. Vanessa Voegele

    Verified customer

    提交于 Apr 28 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (tonsil, intestine)
    Specification tonsil, intestine
    Fixative Formaldehyde
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinase K, 5 min, 37C
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    提交于 Dec 29 2009

    Dear Kol, Thank you for your enquiry. The composition of the Ab15811 (lactoferrin Ab) storage buffer is: PBS, glycerol and stabilizing proteins, pH 7.2-7.6. The buffer contains free amines and therefore can not be used for coupling through...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"