Anti-Lactoferrin抗体(ab15811)
Key features and details
- Rabbit polyclonal to Lactoferrin
- Suitable for: IP, ICC, IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Lactoferrin抗体
参阅全部 Lactoferrin 一抗 -
描述
兔多克隆抗体to Lactoferrin -
宿主
Rabbit -
经测试应用
适用于: IP, ICC, IHC-P, ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) (Human).
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常规说明
Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified using immobilized antigenic proteins and pre adsorbed on an immobilized heterologus lactoferin matrix to remove the cross reacting antibodies. -
Primary antibody说明
Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15811于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ELISA | (1) |
Use at an assay dependent concentration. PubMed: 17244155
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WB | (1) |
1/500. Predicted molecular weight: 79 kDa.
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说明 |
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IP
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. PubMed: 17244155 |
WB
1/500. Predicted molecular weight: 79 kDa. |
靶标
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功能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
序列相似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4057 Human
- Omim: 150210 Human
- SwissProt: P02788 Human
- Unigene: 529517 Human
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别名
- GIG12 antibody
- Growth inhibiting protein 12 antibody
- HLF2 antibody
see all
图片
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Anti-Lactoferrin antibody (ab15811) at 1/500 dilution + Human Lactoferrin
Predicted band size: 79 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab15811 被引用在 20 文献中.
- Kumagai Y et al. Antimicrobial peptide LL-37 ameliorates a murine sepsis model via the induction of microvesicle release from neutrophils. Innate Immun 26:565-579 (2020). PubMed: 32600088
- Mao Y et al. Processed human amniotic fluid retains its antibacterial activity. J Transl Med 17:68 (2019). PubMed: 30823930
- Liu L et al. Assessment of bioactivities of the human milk lactoferrin-osteopontin complex in vitro. J Nutr Biochem 69:10-18 (2019). PubMed: 31048205
- Pérez-Debén S et al. iTRAQ comparison of proteomic profiles of endometrial receptivity. J Proteomics 203:103381 (2019). PubMed: 31102758
- Kahn S et al. Exosomal MicroRNAs in Milk from Mothers Delivering Preterm Infants Survive in Vitro Digestion and Are Taken Up by Human Intestinal Cells. Mol Nutr Food Res 62:e1701050 (2018). PubMed: 29644801