• 产品名称Anti-Lactoferrin抗体
    参阅全部 Lactoferrin 一抗
  • 描述
    山羊多克隆抗体to Lactoferrin
  • 经测试应用适用于: IHC-P, WB, ELISA, ICCmore details
  • 种属反应性
    与反应: Cow
  • 免疫原

    Immunogen is purified lactoferrin of bovine milk origin


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was solid phase adsorbed to ensure specificity. ab112968 was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab112968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/2000.
WB 1/1000 - 1/30000. Predicted molecular weight: 78 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
ELISA 1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1000-1/30000 dilution.
ICC 1/200 - 1/2000.


  • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • 序列相似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Anti-Lactoferrin antibody 图像

  • All lanes : Anti-Lactoferrin antibody (ab112968) at 1/1000 dilution

    Lane 1 : Resuspended cow lactoferrin powder at 2 µg
    Lane 2 : Resuspended cow lactoferrin powder at 1 µg
    Lane 3 : Resuspended cow lactoferrin powder at 0.5 µg
    Lane 4 : Resuspended cow lactoferrin powder at 0.1 µg
    Lane 5 : Resuspended cow lactoferrin powder at 0.05 µg
    Lane 6 : Resuspended cow lactoferrin powder at 0.01 µg

    Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Federico Benetti.

    Blocked with 3% BSA for 1 hour at 20°C.

    Incubated with the primary antibody for 16 hours at 4°C in blocking buffer.

    Membrane - Nitrocellulose.

    See Abreview

Anti-Lactoferrin antibody (ab112968)参考文献

ab112968 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Cow Purified protein (Resuspended cow lactoferrin powder)
Gel Running Conditions Reduced Denaturing (4% stacking 10% resolving)
Loading amount 2 µg
Specification Resuspended cow lactoferrin powder
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Dr. Federico Benetti

Verified customer

提交于 May 02 2017