Anti-Lactate Dehydrogenase isozyme H4抗体(ab81485)

概述

  • 产品名称Anti-Lactate Dehydrogenase isozyme H4抗体
  • 描述
    兔多克隆抗体to Lactate Dehydrogenase isozyme H4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Cat, Dog, Chimpanzee, Zebrafish, Spider Monkey
  • 免疫原

    Synthetic peptide: PLSCHGWVLG EHGDSSVPVW SGMNVAGVSL KTLHPDLGTD KDKEQWKEVH , corresponding to a region within internal sequence amino acids 182 - 231 of Human Lactate Dehydrogenase isozyme H4 (NP_005557)

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab81485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

靶标

  • 相关性Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 19 protein antibody
    • L lactate dehydrogenase A antibody
    • L lactate dehydrogenase A chain antibody
    • Lactate dehydrogenase A antibody
    • LDH A antibody
    • LDH antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH1 antibody
    • LDHA antibody
    • PIG19 antibody
    • Proliferation inducing gene 19 antibody
    • Renal carcinoma antigen NY REN 59 antibody
    • Renal carcinoma antigen NYREN59 antibody
    see all

Anti-Lactate Dehydrogenase isozyme H4 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Lactate Dehydrogenase isozyme H4 with ab81485 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Lactate Dehydrogenase isozyme H4. Right - Merge.


  • Predicted band size : 37 kDa
    Western blot analysis of HeLa cell lysate labeling Lactate Dehydrogenase isozyme H4 with ab81485 at 1.0µg/ml.
  • Anti-Lactate Dehydrogenase isozyme H4 antibody (ab81485) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa

Anti-Lactate Dehydrogenase isozyme H4 antibody (ab81485)参考文献

ab81485 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (A549 Lung cancer cells)
Specification A549 Lung cancer cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
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Dr. Dimitra Kalamida

Verified customer

提交于 Mar 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"